Dr. Hui-Fen Wu

◆Office:理4025

◆Laboratory: 理4030

◆Telephone: 886-7-5252000 ext. 3909

◆FAX: 886-7-5253908

◆e-mail: hwu@faculty.nsysu.edu.tw

◆Laboratory Website

Career

◇Professor, National Sun Yat - Sen University (2006)
◇Professor, Tamkang University (2001)
◇Associate Professor, Tamkang University (1996)
◇Postdoctorate, University of California at Berkeley, USA (1996)
◇PhD., University of Texas at Austin, USA (1994)
◇M.S., University of Texas at Austin, USA (1992)

◇B.S., National Taiwan Normal University (1989)

Research Interests

Our research interests are mainly focussed on applications of nanoparticles and quantum dots combined with matrix assisted laser desorption/ionization mass spectrometry (MALDI/MS) and Electrospray ionization tandem mass spectrometry (ESI/MS/MS) for protein analysis, protein binding interactions and also developing nano materials for biomolecule analysis via liquid phase microextraction. Our Lab exhibits a tradition to accept many post-docs and graduate students from India and English is the main language. Potential Indian post-docs or graduate students with related background or research capability are welcomed to apply.

Topics of current research work:

  1. Using modified nanoparticles prepared in organic solvent as electrostatic or hydrophobic affinity probes and also combined the liquid-phase microextraction (LPME) techniques for separation and preconcentration of proteins in biological samples prior to MALDI-TOF and ESI –ion trap mass spectrometry.

  2. Developing methods for efficient extraction and rapid enrichment of proteins in biological samples by using nanoparticles or quantum dots in MALDI- MS and ESI-MS.

  3. Developing single drop organic nanoprobes for protein analysis in MALDI-MS and ESI-MS by synthesizing surface modification nanoparticles.

  4. Enrichment of mass spectrometric signals by nanomaterials and quantum dots in MALDI and ESI-MS.

  5. Developing nano materials and quantum dots for acceleration of microwave assisted enzamatic digestions of proteins and phosphoproteins.

  6. Analysis of proteins from microorganisms by MALDI and ESI mass spectrometry.

  7. Developing phosphopeptide enrichment techniques by using nanomaterials.

  8. Developing nano materials and quantum dots as the matrix for protein analysis in MALDI-MS.

  9. Probing the binding interactions of nanoparticles and quantum dots with proteins.

Selected Publications

  1. Kamlesh Shrivas, Suresh K. Kailasa, Hui-Fen Wu*, Quantum dots laser desorption/ionization mass spectrometry (QDLDI-MS): multifunctional CdSe quantum dots as the matrix, concentrating probes and acceleration for microwave enzymatic digestion for peptide analysis and high resolution detection of proteins in a linear MALDI-TOF MS, Proteomics, 2009, in press (IF:5.479).

  2. Suresh K. Kailasa, K. Kiran, Hui-Fen Wu*, Comparison of ZnS Semiconductor Nanoparticles Capped with Various Functional Groups as the Matrix and Affinity Probes for Rapid Analysis of Cyclodextrins and Proteins in the SALDI-TOF Mass Spectrometry, Analytical Chemistry, 2008, 80, 9681-9688.(IF 5.287).

  3. Kamlesh Shrivas and Hui-Fen Wu*, Modified Silver Nanoparticle as Hydrophobic Affinity Probes for Rapid Analysis of Peptide and Proteins in Biological Samples by coupling liquid-liquid microextraction with AP-MALDI/ion trap and MALDI/Time of Flight Mass Spectrometry. Analytical Chemistry, 2008, 80, 2583-2589.(IF:5.287).

  4. Kamlesh Shrivas and Hui-Fen Wu*, Oxidized multiwall carbon nanotubes for quantitative determination of cationic surfactants in water samples using AP-MALDI-ion trap Mass Spectrometry. Analytica Chimica Acta. 2008, 628, 198-203 (IF3.186).

  5. Kamlesh Shrivas and Hui-Fen Wu*, Applications of silver nanoparticles capped with different functional groups as the matrix and affinity probes in SALDI-TOF and AP-MALDI- ion trap mass spectrometry for rapid analysis of sulphur drugs and biothiols in human urine, Rapid Communications in Mass Spectrometry, 2008, 22, 2863-2872 (IF 2.971).

  6. P. Sudhir, K Shrivas, Z. C. Zhou, H.-F. Wu*, Single drop microextraction using silver nanoparticles as electrostatic probes for peptide analysis in AP-MALDI/MS and comparison with gold electrostatic probes and silver hydrophobic probes, Rapid Communications in Mass Spectrometry, 2008, 22, 3076-3086 (IF2.971).

  7. Kamlesh Shrivas and Hui-Fen Wu*, Functionalized multiwalled carbon nanotubes as a preconcentrating probe for rapid monitoring of cationic dyestuffs in environmental water using AP-MALDI/MS. Journal of Separation Science, 2008, 31, 3603-3611. (IF2.632).

  8. Kavita Agrawal and Hui-Fen Wu*, Bare silica nanoparticles as concentrating and affinity probes for rapid analysis of aminothiols, lysozyme and peptide mixtures using AP-MALDI/ion trap and MALDI/TOF mass spectrometry, Rapid Communications in Mass Spectrometry, 2008, 22, 283-290.

  9. Chih-Hao Yang, Nadeem Khan, Hui-Fen Wu*, Differentiation and quantification of xylene isomers by combining headspace solid-phase microextraction/gas chromatography and self-ion molecule reaction in an ion trap tandem mass spectrometry , Journal of Separation Science, 2008, 31, 3050-3057.(IF:2.632)

  10. Hui-Fen Wu*, Hsin-Yi Ku, Jyh-Hao Yen, Liquid phase microextraction for rapid AP-MALDI and quantitation of nortriptyline in biological matrices, Journal of Separation Science, 2008, 31, 2288-2294.

  11. Hui-Fen Wu* and Jyh-Hao Yen, Dynamic liquid phase nanoextraction coupled to GC/MS for rapid analysis of methoxyacetophenone and anisaldehye isomers in urine. Journal of Separation Science, 2008, 31, 2295-2302.

  12. Kavita Agrawal, Hui-Fen Wu*, Kamlesh Shrivas, Reverse Micelle-Microextraction as concentrating and affinity probes for rapid analysis of thiopeptide in the AP-MALDI/ion trap and MALDI/TOF Mass Spectrometry. Rapid Communications in Mass Spectrometry, 2008, 22, 1437-1444.

  13. Kamlesh Shrivas and Hui-Fen Wu*, Ultrasonication followed by single drop microextraction combined with GC/MS for rapid determination of organochlorine pesticides from fish. Journal of Separation Science, 2008, 31, 380-386.

  14. Yao-Tang Ke, Hui-Fen Wu*, Applications of functional nanoparticles as the affinity probes for analysis of peptides and proteins by coupling solvent microextraction with the MALDI mass spectrometry, Chemistry (the Chin. Chem. Soc., Taipei), 2008, 66, No3, 231-240.

  15. Kamlesh Shrivas and Hui-Fen Wu*, Quantitative bioanalysis of quinine by AP-MALDI/MS assisted with dynamic drop-to-drop solvent microextraction (Dynamic-DDSME) Analytica Chimica Acta. 2007, 605, 153-158..

  16. Bih-Show Lou, Yuan-Chin Chen and Hui-Fen Wu*, Probing the non-covalent binding interaction of Na+ channel inactivation gate peptide in linker between domain III and IV with 5,5-diphenyhydantoin drug in Electrospray/ Ion Trap Tandem Mass Spectrometry, Rapid Communications in Mass Spectrometry, 2007, 21, 2795-3802.

  17. Kamlesh Shrivas and Hui-Fen Wu*, A simple and rapid quantitative method by direct coupling drop-to-drop solvent microextraction with gas chromatography/mass spectrometry to determine caffeine in one drop of beverages and foods, Journal of Chromatography A, 2007, 1170, 9-14.(IF:3.641)

  18. Kavita Agrawal and Hui-Fen Wu*, Drop-to-drop solvent microextraction (DDSME) coupled with gas chromatography mass spectrometry for rapid determination of trimeprazine in urine and blood of rats: application to pharmacokinetic studies. Rapid Communications in Mass Spectrometry, 2007, 21, 3352-3356.

  19. Shuchen Hsieh*, Hsin-Yi Ku, Yao-Tang Ke, Hui-Fen Wu*, Self-Assembled Monolayer Modified Silicon Substrate to enhance the sensitivity of peptide detection for AP-MALDI Mass Spectrometry. Journal of Mass Spectrometry, 2007, 42, 1628-1636. (IF 3.062).

  20. Kamlesh Shrivas and Hui-Fen Wu*, A Rapid, Sensitive and Effective Quantitative Method for Simultaneous Determination of Cationic Surfactant mixtures from River and Municipal Waste Water by Direct Combination of Single Drop Microextraction with AP-MALDI Mass Spectrometry Journal of Mass Spectrometry, 2007, 42, 1637-1644.(IF:3.062)

  21. Kamlesh Shrivas and Hui-Fen Wu*, Single drop microextraction as a concentrating probe for rapid screening of low molecular weight of drugs from human urine using AP-MALDI Mass Spectrometry. Rapid Communications in Mass Spectrometry, 2007, 21, 3103-3108.

  22. R. Sekar, H. F. Wu*. Quantification method for analysis of monensin in soil, water and urine by direct combining single drop microextraction with atmospheric pressure matrix assisted laser desorption/ionization mass spectrometry. Analytical Chemistry, 2006, 78, 6306-6313.

  23. H. F. Wu*, J. H. Yen, C. C. Chin, Combining drop to drop solvent microextraction with gas chromatography/mass spectrometry using electronic ionization and self-ion/molecule reaction method to determine methoxyacetophenone isomers in one drop of water. Analytical Chemistry, 2006, 78, 1707-1712.

  24. H. F. Wu*, C. H. Lin. Direct combination of immersed single-drop microextraction with atmospheric pressure matrix-assisted laser desorption/ionization tandem mass spectrometry for rapid analysis of a hydrophilic drug via hydrogen-bonding interaction and comparison with liquid-liquid extraction and liquid-phase microextraction using a dual gauge microsyringe with a hollow fiber. Rapid Communications in Mass Spectrometry, 2006, 20, 2511-2515.

  25. B. W. Lai, B. M. Liu, P. Malik, H.-F. Wu*, Combination of Liquid-Phase Hollow Fiber Membrane Microextraction with Gas Chromatography/Negative Chemical Ionization Mass Spectrometry for the Determination of Dichlorophenol Isomers. Analytica Chimica Acta. 2006, 576, 61-66.

  26. N. Rahman, S. Azmi, H. F. Wu, The importance of impurity analysis in pharmaceutical products: an integrated approach, Accreditation and Quality Assurance, Springer Verlag, 2006, 11, 69-74.(IF:0.717)

  27. P. Sudhir, H.-F. Wu*, Z. C. Zhou, identification of peptides using gold nanoparticles-assisted single drop microextraction coupled with AP-MALDI mass spectrometry. Analytical Chemistry, 2005, 77, 7380-7385.

  28. P. Sudhir, H.-F. Wu*, Z. C. Zhou, An application of electrospray ionization tandem mass spectrometry to probe the interaction of Ca+2/Mg+2/Zn+2 and Cl- with gramicidin A, Rapid Communications in Mass Spectrometry, 2005, 19, 1517-1521.

  29. P. Sudhir, H.-F. Wu*, Z. C. Zhou, Probing the interaction of kojic acid antibiotics with iron (III) chloride by using electrospray tandem mass spectrometry, Rapid Communications in Mass Spectrometry, 2005, 19, 209-212.

  30. H. F. Wu*, Developing chemical ionization, host-guest chemistry, self- ion/molecule reaction, single drop microextraction and electrospray mass spectrometry in an ion trap mass spectrometry, Chemistry (the Chin. Chem. Soc., Taipei), 2004, 62, 443-450.

  31. C. H. Yan, H. F. Wu*, A Liquid-Phase Microextraction Method, combining a Dual Gauge Microsyringe with a Hollow Fiber Membrane for the determination of Organochlorine Pesticides in Water by Gas Chromatography/Ion Trap Mass Spectrometry. Rapid Communications in Mass Spectrometry, 2004, 18, 3015-3018.

  32. N. Khan, H.-F. Wu*, Analysis of silymarin extracted from commercial dosage forms by combining liquid-liquid extraction with negative electrospray tandem mass spectrometry. Rapid Communications in Mass Spectrometry, 2004, 18,2960-2962.

  33. B. M. Liu, P. Malik, H. F. Wu*, Single-Drop Microextraction and Gas Chromatography-Mass Spectrometric Determination of Anisaldehyde Isomers in Human Urine and Blood Serum. Rapid Communications in Mass Spectrometry, 2004, 18, 2059-2064.

  34. H. F. Wu*, P. Y. Lin, Ethylenediamine as a liquid reagent to probe the hydrogen - bonding and host guest interaction with crown ethers in an Ion Trap Tandem Mass Spectrometer, Rapid Communications in Mass Spectrometry, 2004, 18, 1365-1373.

  35. H. F. Wu*, C. H. Chen, M. T. Wu, Observation of Self - Ion - Molecule Reaction during collisionally activated dissociation in an ion - trap mass spectrometer, Journal of Mass Spectrometry, 2004, 39, 396-401.

  36. L. Brewer, K. Krushwitz, R. Lamoreaux, H.F. Wu, Properties of valuable new intermetallics: Brewer–Engel model applied to the bonding of transition metal dialuminides, Intermetallics, 2003, 11, 1103-1109.

  37. H. F.Wu*, W. F. Wu, Comparing differentiation of xylene isomers by electronic ionization, chemical ionization and Self-Ion/Molecule Reactions and the first observation of methyne addition ions for xylene isomers in Self-Ion/Molecule Reactions for non-nitrogenated compounds, Rapid Communications in Mass Spectrometry, 2003, 17, 2399-2406.

  38. H. F. Wu*, C. H. Chen, L. C. Lu, Probing the Reaction Mechanisms of Self - Ion/Molecule Reaction for Dopamine in an Ion Trap Mass Spectrometer, Rapid Communications in Mass Spectrometry, 2003, 17, 1479-1482.

  39. H. F. Wu*, P. Y. Lin, Probing the Effects of Reagent Gas Pressure and Ion Source Temperature for Dimethyl Ether Chemical Ionization of Tricyclic Antidepressants in an External Source Ion Trap Mass Spectrometer, J. Chin. Chem. Soc., 2003, 50, 1251-1257.

  40. H. F. Wu*, Y. J. Chuan, Isomer Differentiation by Combining Gas Chromatography, Selective Self–Ion/ Molecule Reaction and Tandem Mass Spectrometry in an Ion Trap Mass Spectrometer, Rapid Communications in Mass Spectrometry, 2003, 17,1030-1036.

  41. H. F. Wu*, Y. R. Chen, B. W. Lai, Novel observation of total ion chromatogram (TIC) splitting under positive chemical ionization in an external source Ion Trap Mass Spectrometer, Journal of Mass Spectrometry, 2003, 38,458-459.

  42. H. F. Wu, L. C., Lu, 2002年諾貝爾化學獎－質譜儀分析技術的突破，開展生化科技新領域, 科學發展月刊, 2003, 361, 50-53.

  43. H. F. Wu*, S. M. Huan, C. F. Wu, 2002, Conformational Analysis and Binding Affinity Determination for Host-Guest Complexation of Alkali Metal Ion with Bis (Crown Ether)s by Electrospray Mass Spectrometry and Molecular Modeling, European Journal of Mass Spectrometry, 2002, 8, 375-380.

  44. H. F. Wu*, C. H. Chen, Hydroxide and oxygen atom attachment to dichlorophthalic anhydride in negative ion chemical ionization with collisionally activated dissociation in an external source ion trap mass spectrometer, European Journal of Mass Spectrometry, 8, 329-332.

  45. Hui-Fen Wu*, Li-Wei Chen and Chien-Hung Chen, 2001, Probing the Reactive Sites for Ion/Molecule Reactions of Anthraquinones with Dimethyl Ether by an External Source Ion Trap Tandem Mass Spectrometer and Computational Chemistry, Rapid Communications in Mass Spectrometry, 15, 1977-1987.

  46. Hui-Fen Wu* and Ming-Yi Ho,“Selective Self - Ion / Molecule Reactions in Both External and Internal Source Ion Trap Mass Spectrometers”, Rapid Communications in Mass Spectrometry, 2001, 15, 1309-1316.

  47. Hui-Fen Wu*, Chien-Hung Chen and Ming-Yi Ho, 2001, April,“Examination of the Best Pressure Range for Ion/Molecule Reactions of Anthraquinones in an External Source Ion Trap Mass Spectrometer” Analytical Sciences, 17, 515-518.

  48. Hui-Fen Wu*, Li-Wei Chen, Jhen-Chen Wang and Ya-Ping Lin, 2001, February,“Simulation of the Collisional Cooling Effect for Binary and Ternary Buffer Gas Mixtures in a Quadrupole Ion Trap Mass Spectrometer”. European Journal of Mass Spectrometry, 7, 1-6.

  49. Hui-Fen Wu*, 2000, August, “Study of Temperature and Pressure Effect of Negative Chemical Ionization Mass Spectrometry Using Methane and Oxygen as Reagent Gases in an External Source Ion Trap Mass Spectrometer”, Journal of Mass Spectrometry, 35, 1049-1050.

  50. Hui-Fen Wu* and Ya-Ping Lin, 2000, June, “Study of Ion-Molecule Reactions and Collisionally Activated Dissociation of Dopamine and Adrenaline by an Ion Trap Mass Spectrometer with an External Ionization Source. European Journal of Mass Spectrometry, 6, 65-77.

  51. Hui-Fen Wu*, Li-Wei Chen and Ya-Ping Lin, 1999, December, “Simulation of Collisional Cooling Effect in a Quadrupole Ion Trap Mass Spectrometer”. Journal of the Chinese Chemical Society, 46, 923-932.

  52. Hui-Fen Wu* and Ya-Ping Lin, 1999, December, “Determination of Sensitivity of the External Source Ion Trap Tandem Mass Spectrometer Using Dimethyl Ether Chemical Ionization”. Journal of Mass Spectrometry, 34, 1283-1285.

  53. Hui-Fen Wu*and Li-Wei Chen, 1998, October, “Study of Host-Guest Complexation of Alkaline Ion, Aluminum Ion and Transition Metal Ions with Crown Ethers by Fast Atom Bombardment Mass Spectrometry”. Journal of the Chinese Chemical Society, 45, 689-699.

  54. L. Brewer and H.- F. Wu,1997, “Parameters for Calculation of Properties of Acid-Base Intermetallics”. Proc.-Electrochem. Soc., Volume: 97-39, number: High Temperature Materials Chemistry, p 60-69, (CA:128(7)80209z).

  55. H.-F. Wu, Leo Brewer, 1997, January, “Calculation of Thermodynamic Effect of the Brewer-Engel Generalized Acid - Base Reactions of the 1:1 Intermetallics for Nontransition Metals Al, Mg with Transition Metals”. Journal of Alloys and Compounds, 247,1-8 (SCI).

  56. E.J. Alvarez, H.-F. Wu, C.-C. Liou and J. S. Brodbelt, 1996, September, “Collisionally Activated Dissociation of Transition Metal Ion/Polyether Complexes in a Quadrupole Ion Trap”. Journal of the American Chemical Society, 118, 9131-9138.

  57. H.-F. Wu, Leo Brewer, 1996, February, “Calculation of Binary Phase Diagrams of Refractory Metals Ta, W, Tc and Re with Liquid Metals Am, Cm, and Bk by Using a Modification of Regular Solution Theory”. Journal of Phase Equilibria, 17,36-39.

  58. C.-C.-Liou, J. Isbell. H.-F. Wu. J. S. Brodbelt, R. A. Bartsch, J. C. Lee and J. L. Hallman, 1995, April, “Structually- Selective Gas-Phase Ion-Molecule Reactions of Dibenzo-16-Crown-5 Compounds” Journal of Mass Spectrometry, 30,572-580.

  59. H .-F. Wu, J. S. Brodbelt, 1995, February , “Gas-Phase Chelation Reactions of Monopositive Cations with Heteroaromatic Ligands”. Inorganic Chemistry, 34, 615 –621.

  60. H.-F. Wu, J. S. Brodbelt , 1994, October, “Influence of Heteroatom Donors on the Orders of Relative Gas-Phase Binding Affinities of Macrocyclic Polyethers”. Journal of Inclusion Phenomena and Molecular Recognition in Chemistry , 18, 37-44.

  61. H.-F. Wu, J. S. Brodbelt , 1994, July, “The Gas-Phase Complexation of Monovalent Alkaline Earth Metal Ions with Polyethers: Comparision to Alkali Metal Ion and Aluminum Ion Complexation”. Journal of The American Chemical Society, 116,6418-6426.

  62. C.-C .Liou, H.-F. Wu , J. S. Brodbelt ,“Hydrogen-Bonding Interactions in Gas-Phase Polyether/Ammonium Ion-Complexes”. Journal of The American Society for Mass Spectrometry, 1994, 5, 260-273.

  63. H.-F. Wu, J. S. Brodbelt, “Comparison of the Orders of Gas-Phase Basicities and Ammonium Ion Affinities of Polyethers by the Kinetic Method and Ligand Exchange Technique.” Journal of The American Society for Mass Spectrometry, 1993, 4,718-722.

  64. H.-F. Wu, J. S. Brodbelt,“Effects of Reaction Ion Kinetic Energy on Both Endothermic and Exothermic Ion/Molecule Reactions in a Quadrupole Ion Trap Mass Spectrometer.” International Journal of Mass Spectrometry and Ion Processes, 1993, 124,175-184.

  65. H.-F. Wu, J. S. Brodbelt,“Effects of Collisional Cooling on Ion Detection in a Quadrupole Ion Trap Mass Spectrometer”. ICR/Ion Trap Newsletter, 1992, 25,22-24.

  66. H.-F. Wu, J. S. Brodbelt, “Effects of Collisional Cooling on Ion Detection in a Quadrupole Ion Trap Mass Spectrometer”. International Journal of Mass Spectrometry and Ion Processes, 1992, 115,67-81.