謝建台 教授 (Prof. Shiea Jentaie)

簡介

謝建台教授於1980年畢業於中興大學化學系,19881991年分別取得美國蒙大拿州立大學 (Montana State University, MSU) 有機地球化學碩士及分析化學博士學位,隨後赴賓州州立大學 (Pennsylvania State University, PSU) 材料科學系擔任博士後研究,再於1991年回到國立中山大學化學系任教迄今。

研究方向主要有三:
(1)新大氣壓力質譜游離源之開發,以及其在食品安全、鑑識科學、環境化學、工業化學、醫藥學、反恐、及動植物化學等相關領域之應用
(2)高效液相層析、氣相層析、毛細管電泳、以及薄層層析-質譜介面的開發
(3)以現代質譜法快速偵測生物體液中之各式疾病生物指標以及應用質譜分子影像技術於生物醫學科學

Office: C3018
Lab: C2004
Tel: +886-7-5252000 ext.3933
Fax: +866-7-5253933
E-mail: jetea@mail.nsysu.edu.tw




過去十年來,他積極參與並致力於推動台灣以及亞洲區的質譜研究,曾擔任世界質譜基金會 (International Mass Spectrometry Foundation, IMSF) 執行理事兼亞太區代表及台灣質譜學會會長。他從事質譜研究多年,曾獲得多項國內外學術研究獎項如:2009年行政院國家科學委員會所頒發之『傑出研究獎』、2010年行政院經濟部頒發之『國家創作發明獎』、2011年中國化學會頒發的『化學技術獎章』、2012年由遠東集團有庠基金會所頒發之『有庠科技發明獎』、2013年由美國質譜學會所頒發之『傑出論文審查人獎』、由台灣質譜學會所頒發之『優秀質譜研究學者獎』以及 2017 年由行政院科技部所頒發之『傑出技術移轉貢獻獎』。在國立中山大學服務迄今,分別於 20022006 2009年獲得『國立中山大學研究傑出獎』,以及 200720112017年的『中山發明獎』及『產學傑出獎』等獎項。2010年也獲英國皇家化學會頒發英國皇家化學會會士頭銜。他也曾擔任多項政府單位之審查、審議及諮議委員,也是國立中山大學學術研究及產學研究兩項之特聘教授,除此之外現在也是 J. Am. Soc. Mass Spectrom. (JASMS),  Clin. Mass Spectrom. (CMS), Mass Spectrom., Mass Spectrom. Let., Int. J. Mass Spectrom. (IJMS), Rapid Commun. Mass Spectrom. (RCM), Anal. Methods, Technology, Clin. Chim. Acta (CCA), J. Food Drug Anal. (JFDA) 以及 Current Chromatogr. 等專業國際學術期刊的編輯委員或諮詢委員會成員。
 
 
15秒救人!農業毒物檢測新方法

學歷

  1. 國立中興大學化學學士 (1977-1981)
  2. 美國蒙大拿州州立大學化學碩士 (有機地球化學) (1985-1988)
  3. 美國蒙大拿州州立大學化學博士 (分析化學) (1988-1991)

學術經歷與服務

  1. 美國賓州州立大學材料科學系博士後研究 (1991-1991)
  2. 美國蒙大拿州州立大學化學及生化學系訪問副教授 (1995-1996)
  3. 美國加州大學洛杉磯分校化學及生化學系訪問教授 (2004-2005)
  4. 國立中山大學化學系副教授 (1991-1998)
  5. 國立中山大學化學系教授 (1998-迄今)

 

  1. 國科會自然處審議委員 (2000-2002)
  2. 國科會自然處諮議委員 (2003-2005)
  3. 國科會自然處諮議委員 (2010-2012)
  4. 台灣質譜學會理事長 (2009-2012)
  5. 行政院公共工程委員 (2009-迄今)
  6. 世界質譜基金會執行理事兼亞太區代表 (2009-2016)
  7. 世界質譜基金會 Thomson Medal Award Committee 主席 (2011-2012)
  8. 世界質譜基金會 Curt Brunnée Award Committee主席 (2013-2014)
  9. 世界質譜基金會執行理事兼執行祕書 (2014-2015)
  10. 國立中山大學理學院副院長 (2009-2011)
  11. 國立中山大學跨領域科學研究中心主任 (2009-2011)
  12. 國立中山大學科技醫學研究中心主任 (2011-2012)
  13. 國立中山大學毒藥物暨生醫快篩科技研究中心主任 (2018-迄今)

 

  1. Anal. Methods (RSC)副主編 (2009-2012)
  2. Anal. Methods (RSC)編輯委員 (2013-迄今)
  3. Mass Spectrom. Let. (KSMS)編輯委員 (2011-2013)
  4. Int. J. Mass spectrom. (Elsevier)諮詢委員會成員 (2012-迄今)
  5. Current Chromatogr. (Bentham Science)編輯委員 (2012-迄今)
  6. Mass Spectrom. (Wiley)編輯委員 (2012-迄今)
  7. Technology (World Scientific Publishing Company)編輯委員 (2013-迄今)
  8. Clin. Mass Spectrom. (Elsevier)諮詢委員會成員 (2016-迄今)
  9. J. Am. Soc. Mass Spectrom. (Springer)編輯委員 (2017-迄今)
  10. Clin. Chim. Acta. (Elsevier)專刊編輯委員 (2012-2013)
  11. Mass Spectrom. (Wiley)專刊編輯委員 (2013-2014 & 2016-2017)
  12. J. Food Drug Anal. (Elsevier)專刊編輯委員 (2018-2019)
  13. Rapid Commun. Mass Spectrom. (Wiley)專刊編輯委員 (2019-2020)

獎勵與榮譽

  1. 最佳碩士論文獎,美國蒙大拿州州立大學 (1988)
  2. 會議壁報論文首獎,世界人類蛋白質體會議 (HUPO) (2006)
  3. 傑出研究獎,行政院國家科學委員會 (2009)
  4. 英國皇家化學會會士 (FRSC)英國化學會 (2010-迄今)
  5. 國家創作發明獎,行政院經濟部 (2010)
  6. 化學技術獎章,中國化學年會 (2011)
  7. 有庠科技發明獎,遠東財團法人徐有庠先生紀念基金會 (2012)
  8. 優秀質譜研究學者獎,台灣質譜學會 (TSMS) (2013)
  9. 傑出論文審查人獎,美國質譜學會 (ASMS) (2013)
  10. 儀器精品獎,第八屆高雄國際儀器展 (2015)
  11. 傑出技術移轉貢獻獎,行政院科技部 (2016)
  12. 未來科技突破獎,行政院科技部 (2017)
  13. 化學最佳論文獎,中國化學會 (2018)
  14. 台灣質譜學會獎章,台灣質譜學會 (TSMS) (2019)
  15. 未來科技突破獎,行政院科技部 (2019)
  16. 傑出研究獎,行政院科技部 (2020)

 

  1. 理學院傑出教學獎,國立中山大學 (2001)
  2. 研究傑出獎,國立中山大學 (2002)
  3. 研究傑出獎,國立中山大學 (2006)
  4. 中山發明獎,國立中山大學 (2007)
  5. 研究傑出獎,國立中山大學 (2009)
  6. 產學傑出獎,國立中山大學 (2011)
  7. 特聘教授 (產學類),國立中山大學 (2011)
  8. 特聘教授 (學術研究類),國立中山大學 (2011-2014)
  9. 特聘教授 (產學類),國立中山大學 (2014)
  10. 特聘教授 (學術研究類),國立中山大學 (2014-2017)
  11. 產學傑出獎,國立中山大學 (2017)
  12. 特聘教授 (產學類),國立中山大學 (2017)
  13. 產學研究績優教師獎,國立中山大學 (2018)
  14. 學術研究績優教師獎,國立中山大學 (2018)
  15. 產學研究績優教師獎,國立中山大學 (2019)
  16. 西灣講座,國立中山大學 (2020)

主辦國內/外會議

  1. 2013.11.01~11.02 質譜分析技術在臨床醫學檢驗研討會 (2013 Mass Spectrometry for Clinical Diagnosis)
  2. 2014.11.07~11.08 質譜分析技術在臨床醫學檢驗研討會 (2014 Mass Spectrometry for Clinical Diagnosis)
  3. 2015.11.05~11.07 質譜分析技術在臨床醫學檢驗研討會 (2015 Mass Spectrometry for Clinical Diagnosis)
  4. 2016.06.27~06.29 第十三屆台灣質譜學會年會暨學術研討會 (The 13th Taiwan Society for Mass Spectrometry Annual Conference)
  5. 2017.03.27 第一屆快篩技術暨應用研討會 (The 1st Conference on Rapid Screening Technologies and Applications)
  6. 2017.06.12~06.13 質譜在食品安全分析之應用研討會
  7. 2017.10.12~10.14 質譜分析技術在臨床醫學檢驗研討會 (2017 Mass Spectrometry for Clinical Diagnosis)
  8. 2018.10.25~10.27 質譜分析技術在臨床醫學檢驗研討會 (2018 Mass Spectrometry for Clinical Diagnosis)
  9. 2019.10.17~10.18 食品安全教育/食品安全暨藥物分析之質譜新技術與策略發展研討會 (2019 Symposium for Food Safety Education/Symposium for New Mass Spectrometric Technologies and Strategies for Food Safety and Drug Analysis)


國際會議邀請演講

2020

  1. Invited Speaker, 8th International Singapore Lipid Symposium Online, Mar. 2020, Signgapore
  2. Chair and Invited Speaker, 2020 Asia-Oceania Mass Spectrometry Conference, Jan. 2020, Macau, China

2019

  1. Plenary Speaker, 2019 Mass Spectrometry for Clinical Diagnosis, Sep. 2019, Ningbo, China
  2. Invited Speaker, 2019 The Japan Society of Analytical Chemistry, Sep. 2019, Chiba Ken, Japan

2018

  1. Plenary Speaker, 2018 Asia HPTLC, Nov. 2018, Bangkok, Thailand
  2. Invited Speaker, 2018 China Mass Spectrometry Conference, Nov. 2018, Guangzhou, China
  3. Keynote Speaker, National Conference on Organic Mass Spectrometry, Sep. 2018, Kaifeng, China
  4. Session Chair, The 22nd International Mass Spectrometry Conference, Aug. 2018, Florence, Italy
  5. Plenary Speaker, The 5th Ambient Ionization Mass Spectrometry, Apr. 2018, Xian, China
  6. Invited Speaker, 14th Asian Conference on Analytical Sciences, Apr. 2018, Jakarta, Indonesia
  7. Invited Speaker, 2018 Mass Spectrometry: Application for Clinical Laboratory, Jan. 2018, Palm Spring, USA

2017

  1. Keynote Speaker, 2017 AOMSC, Dec. 2017, Singapore
  2. Invited Speaker, 3rd National symposium on Mass Spectrometry, Dec. 2017, Xia-Men, China
  3. Invited Speaker, HPLC 2017 Jeju, Nov. 2017, Jeju Island, Korea
  4. Invited Speaker, HPTLC 2017, Jul. 2017, Berlin, Germany
  5. Invited Speaker, PITTCON 2017, Mar. 2017, Chicago, USA
  6. Invited Speaker, 2017 Mass Spectrometry: Application for Clinical Laboratory, Jan. 2017, Palm Spring, USA

2016

  1. Invited Speaker, International Conference Global Food Safety & Antimicrobial Resistance, Nov. 2016, Shenzhen, China
  2. Invited Speaker, 2nd International Conference on Innovations in Mass Spectrometry, Nov. 2016, Moscow, Russia
  3. Chairman and Plenary Speaker, the Ambient Ionization Mass Spectrometry 2016 Conference, Nov. 2016, Guangzhou, China
  4. Invited Speaker, Biological Mass Spectrometry Symposium 2016, Oct. 2016, Tokyo, Japan
  5. Keynote Speaker, 2016 Korea Society for Mass Spectrometry, Aug 2016, Seoul, Korea
  6. Plenary Speaker, Hong Kong Society for Mass Spectrometry 2016, Jun. 2016, Hong Kong
  7. Invited Speaker, World Chinese Mass Spectrometry Conference 2016, Jun. 2016, San Antonio, U.S.A
  8. Invited Speaker, PITTCON 2016, Mar. 2016, Atlanta, U.S.A
  9. Invited Speaker, Mass Spectrometry: Applications to the Clinical Lab 2016, Feb. 2016, Palm Spring, U.S.A

2015

  1. Invited Speaker, 5th BCEIA, Oct. 2015, Beijing, China
  2. Invited Speaker, HPLC 2015, Sep. 2015, Beijing, China
  3. Invited Speaker, Chinese Chemical Society Second National Symposium on Mass Spectrometry, Sep. 2015, Hangzho, China
  4. Invited Speaker, Mass Spectrometry: Applications to the Clinical Lab 2015, 28th March~1st April 2015, San Diego, U.S.A

2014

  1. Invited Speaker, the 17th International Conference & Exhibition on Analysis & Testing of Metallurgical Process & Materials, Jul. 2014, Beijing, China
  2. Invited Speaker and Session Chair, the 5th Asia and Oceania Mass Spectrometry Conference, Jul. 2014, Beijing, China
  3. Keynote Speaker and Session Chair, 2014 HPTLC symposium, Jul. 2014, Lyon, France
  4. Invited Speaker, PITTCON-2014, Mar. 2014, Chicago, USA

2013

  1. Lecturer, 1st International Mass Spectrometry School, Siena University, Sep. 2013, Siena, Italy
  2. Invited Speaker, 1st International Conference on Urine Omics (URINOMICS 2013), Sep. 2013, Lisbon, Portugal
  3. Invited Speaker and Session Chair, 12th Asian Conference on Analytical Sciences (ASIANALYSIS XII), Aug. 2013, Fukuoka, Japan
  4. Lecturer, International Congress for Innovation in Chemistry (PERCH-CIC Congress VIII), May 2013, Pattaya, Thailand
  5. Invited Speaker, The International Symposium on SIMS and Related Techniques Based on Ion-Solid Interaction 2013,Seikei University, Apr. 2013, Tokyo, Japan

2012

  1. Invited Speaker, Department of Biochemistry and Biophysics, Stockholm University, Nov. 2012, Sweden
  2. Session and Workshop Chairs, the 29th International Mass Spectrometry Conference, Sep. 2012, Kyoto, Japan
  3. Invited Speaker, Centre an0d Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Aug. 2012, Hong Kong
  4. Keynote Speaker and Session Chair, Desorption 2012, Jun. 2012, Castle of Rauischholzhausen, Marburg, Germany
  5. Tutorial Lecturer and Invited Speaker, the 60th American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics, May 2012, Vancouver, Canada
  6. Invited Speaker, the 1st Analytix-2012, Mar. 2012, Beijing, China
  7. Invited Speaker, PITTCON-2012, Mar. 2012, Orlando, USA

2011

  1. Plenary Speaker, the 12th International Conference of Ethnopharmacology, Feb. 2012, Kolkata, India
  2. Invited Speaker, Molecular Imaging Workshop, Dec. 2011, Moscow, Russia
  3. Invited Speaker, the 14th Annual Conference of Indian Society for Mass Spectrometry (ISMAS), Nov. 2011, Munnar, India
  4. Invited Speaker, International Conference on Chemistry of Phytopotentials: Health, Energy and Environmental Perspectives (CPHEE 2011), Nov. 2011, Agra, India
  5. Invited Speaker, the 37th International Conference of High Performance Liquid Chromatography, Oct. 2011, Dalian, China
  6. Invited Speaker, the 14th Beijing Conference and Exhibition on Instrumental Analysis (BCEIA), Oct. 2011, Beijing, China
  7. Session and Workshop Chairs, the 2nd Asia and Oceania Mass Spectrometry Conference, Aug. 2011, Busan, Korea
  8. Invited Speaker, Laboratory of Organic Chemistry, Eldgenossische Technische Hochschule (ETH), Jun. 2011, Zurich, Switzerland
  9. Session Chair, the 4th International High Performance Thin Layer Chromatography Symposium, Jun. 2011, Basel, Switzerland
  10. Invited Speaker, the 18th Secondary Ion Mass Spectrometry (SIMS) Symposium, May 2011, Baltimore, USA

2010

  1. Session Chair and Invited Speaker, the 26th Asilomar Conference, American Society for Mass Spectrometry, Oct. 2010, Asilomar, CA, USA
  2. Keynote Speaker, 2012 Taiwan-China Bilateral Analytical Chemistry Symposium, Sep. 2010, Dalian, China
  3. Plenary Speaker, the 1st AOMSC, Jun. 2010, Tsukuba, Japan
  4. Invited Speaker, Institute of Chemistry, Beijing Univ., Jul. 2010, Beijing, China
  5. Keynote Speaker, the 3rd World Chinese Mass Spectrometry Conference, Jul. 2010, Chan-Chun, China

研究興趣

儀器開發 (Instrumentation)


低溫大氣壓游離法 (Low Temperature Ionization)   直接電噴灑游離探針 (Direct Electrospray Probe, DEP)
123
此技術是將具熱不穩定性及高揮發性之化合物溶於低溫的有機溶劑來抑制其化學活性,並通過低溫管路導入到 ESI 游離源或是二次離子源中進行游離。相較於傳統分析法,此技術可得更佳的不穩定分析物離子訊號。


J. Mass Spectrom. (1996, 31, 464-471)
Anal. Chem. (1996, 68, 1062-1066)
  111
此技術是施加高電壓於一上面置有一微滴之樣品溶液之金屬絲上,金屬絲表面可以是經修飾(如奈米氧化鎢管) 或不經修飾 (如銅絲)。樣品液滴在受到高電場的影響下,會移向質量分析器並在金屬絲尖端產生微電噴灑現象。此技術因組裝簡單,使用材料便宜,樣品更換容易,因此未來可應用於大量樣品數目的分析。


Anal. Chem.(1999, 71, 4413-4417)
Rapid Commun. Mass Spectrom. (2003, 17, 1709-1713)
Anal. Chem. (2005, 77, 8170-8173)
Rapid Commun. Mass Spectrom. (2017, 31, 1-9)

                                                                                                                                                                                             

瞬間熱裂解 (Pyrolysis, Py) 結合融合微滴電噴灑游離法 (Fused-droplet electrospray ionization, FD-ESI)   電噴灑輔助雷射脫附游離法 (Electrospray-Assisted Laser Desorption Ionization, ELDI)
789
FD-ESI 是我們實驗室所開發出來的大氣壓力游離技術。我們發現在將 Py 結合 FD-ESI 後,我們可以快速偵測到來自高分子材料的熱裂解極性產物,以此方法,我們可以很容易區分各式不同高分子材料。我們發現儘管蛋白質溶液中含有大量的有機或是無機鹽類,但均可在不經任何樣品前處理步驟下,以FD-ESI/MS 直接得到蛋白質離子的訊號。和傳統的ESI/MS 分析結果比較起來,此技術對鹽類的容忍度是前者的100倍以上。
 
 
Anal. Chem.
(2001, 73, 4983-4988)
J. Mass Spectrom. (2005, 40, 413-415)
Anal. Chem. (2005, 77, 8170-8173)
J. Proteome Res. (2005, 4, 606-612)
  444
原理是先以雷射將分析物分子脫附後,再將這些氣相分子導入一以溶劑進行電噴灑區域內,使分析物分子和電噴灑區內之帶電荷溶劑物種反應而產生游離。


Rapid Commun. Mass Spectrom. (2005, 19, 3701-3704)
Anal. Chem. (2008, 80, 6995-7003)
J. Proteome Res. (2006, 5, 1108-1116)
Anal. Chem. (2008, 80, 4845-4852)
Anal. Chim. Acta
(2019, 1066, 69-78)
J. Food Drug Anal.
(2019, 27, 415-427)
 
    
 
                                                                     
薄層層析法 (Thin Layer Chromatography, TLC) 結合電噴灑輔助雷射脫附游離法 (Electrospray-Assisted Laser Desorption Ionization, ELDI)   雷射誘導聲波脫附 (Laser-Induced Acoustic Desorption, LIAD) 結合融合微滴電噴灑游離法 (Fused-Droplet Electrospray Ionization, FD-ESI)
654 
薄層層析法 (Thin Layer Chromatography, TLC) 具有操作簡單、成本低廉、樣品需求量少的特性,且已被廣泛應用在小分子的分析上,與電噴灑的結合可提供直接且快速地沖提物檢測。而電噴灑輔助雷射脫附游離法 (Electrospray Laser Desorption Ionization, ELDI) 可在一大氣壓力下,不用複雜的樣品前處理,就直接對樣品進行快速的分析。本研究是利用市售樂高模組具可輕易組裝的優點,並與輸送帶結合,發展成一套高通量且可快速篩選各式成分的TLC-ELDI/MS偵測系統,可針對大量樣品包含藥物、化妝品等進行高效率的分析。


Anal. Chem. (2003, 75, 2493-2498)
Anal. Chem. (2012, 84, 5864-5868)
  987 
我們將蛋白質溶液塗敷於一鋁金屬薄片上,鋁膜另一面再以雷射照射,以產生震波造成蛋白質分子的脫附,這些被脫附之蛋白質分子在移動到一電噴灑區後,會進一步被電噴灑所游離。我們發現不論樣品是以固體或是液體,均可以此法成功被游離。


Anal. Chem. (2009, 81, 868-874)
Anal. Chem. (2009, 81, 9274-9281)
 


 


熱脫附電噴灑游離質譜法 (Thermal Desorption Electrospray Ionization, TD-ESI)   雙電噴灑及大氣壓化學游離法 (Dual Electrospray and Atmospheric Pressure Chemical Ionization)
999 
熱脫附電噴灑游離源技術包含了熱脫附裝置和電噴灑離子源,利用一金屬取樣探針沾取或刮取樣品,可於開放空間下分析固體、液體、凝膠及膏狀等型態的樣品,經由加熱區以高溫加熱脫附樣品,並以預先加熱的氮氣作為載流氣體,將分析物攜帶至電噴灑噴霧中產生游離反應,全程只需約 30 秒便可知道結果。具有方便、簡單、快速、即時且感度高等優點。此分析技術提供不同領域的分析者檢測多種不同基質的樣品或於其表面做取樣分析;如尿液中安非他命系列的檢測。


Anal. Chem. (2013, 85, 8956-8963)
  11 
開發了結合電噴灑游離(ESI)和大氣壓化學游離(APCI)的雙游離源,同時電離極性和非極性化合物。將高直流電壓施加到在熔融石英毛細管中流動的甲醇溶液中,在以毛細管尖端處產生ESI電噴灑。將高交流電壓施加到連接到玻璃管的環形電極,在玻璃管與不銹鋼管柱間流動的氮氣產生電漿。由於ESIAPCI所需的高電壓是獨立應用和控制的,因此雙離子源可以在僅ESI,僅APCIESI + APCI模式下運行。通過用連續波長激光或脈衝激光束照射樣品表面將分析物引入ESIAPCI流中。通過將雙ESI+APCI向樣品表面以進行脫附和游離,也可以產生分析物離子。雙離子源中涉及的游離機制包括潘寧電離,離子分子反應和熔滴電噴霧電離。


Anal. Chem. (2015, 87, 1743-1748)



火焰誘導大氣壓化學游離法 (Flame-Induced Atmospheric Pressure Chemical Ionization, FAPCI)   多通道電噴灑游離法 (Multiple Channel Electrospray Ionization)
14
火焰誘導的大氣壓化學游離(FAPCI)是一種溶劑和高電壓組合的 APCI 技術。它利用火焰產生帶電物質,與分析物反應進行游離,並從有機化合物中生成完整的分子離子,與較少的碎片離子。在這項研究中,脫附 FAPCI/MS 被開發用於快速偵測液態,糊狀物和固態的熱穩定有機化合物。通過加熱的噴灑器將液體樣品引入離子源,並且在加熱的噴霧器中形成的分析物與游離源中的帶電物質反應。對於糊狀物和固體樣品分析,會將樣品放置於游離源的火焰附近以進行熱脫附和游離。


J. Am. Soc. Mass Spectrom. (2017, 28, 1473-1481)
Mass. Spectrom. (2017, 6, S0065)

Anal. Chem. (2016, 88, 5159-5165)
Rapid Commun. Mass Spectrom. (2016, 30, 890-896)

  13 
該工作利用連接到多通道電噴灑質譜的流動熱解器檢測高反應性熱解產物環戊二烯基烯酮,環己二烯基烯烴亞胺和乙酰基乙烯酮的質子化分子離子。在流動熱解器中產生的乙烯酮通過氮氣流直接導入七通道電噴霧電離源的中心通道。 同時,通過外部六個通道電噴射含有0.1%三氟乙酸的甲醇溶液。質子化的甲醇離子和由外部六個電噴霧通道產生的帶電液滴促進中性烯酮通過離子-分子反應或吸收隨後質子化而游離。


J. Mass. Spectrom. (1997, 32, 247-250)
Anal. Chem. (1998, 70, 2757-2761)
Anal. Chem. (2000, 72, 1175-1178)


旋轉式電噴灑游離法 (Rotating Electrospray Ionization, RESI)   鋸齒狀薄層層析法結合電噴灑游離質譜法 (Sawtooth TLC-ESI/MS)
21
旋轉電噴灑游離質譜法 (Rotating Electrospray Ionization, RESI) 可用來有效提升離子-分子反應 (Ion-Molecule Reaction, IMR) 的效率。其是藉由機動式馬達將不斷噴出帶電液滴的電噴灑游離源進行高速旋轉,並形成一均勻分布的電噴灑噴霧區域,而此現象特別有益於二段式大氣壓力游離質譜法如 FD-ESI, ELDILIAD-ESI 等技術。除此之外,此技術更可運用在多管式電噴灑游離源上,藉以產生一均勻分布的多管式電噴灑噴霧區域,以克服靜止式因電場干擾所造成的電噴灑不穩定及電噴灑噴霧不均勻等問題。


US8,258,469B2
(美國專利證號)
5193329 (日本專利證號)
1614741 (中國專利證號)
2367197 (歐盟專利證號)

  19
開發了一種簡單、便宜的薄層層析 (TLC) 與電噴灑游離質譜 (ESI/MS) 連接設計,用於掃描和鑑定經TLC分離的化合物。將TLC片修剪成兩個鋸齒狀的TLC試片,再以鋸齒狀三角形尖端面對質譜入口,於尖端加一滴甲醇和高電壓以誘導電噴灑的形成,進行試片上分析物的分析。該系統可連續進行高通量分析,在分析第一個尖端中的分析物後,可藉由自動載台移動TLC試片,進行下一個尖端分析物的分析。


Anal. Chim. Acta (2018, 434, 264-271)
Anal. Chim. Acta (2019, 1049, 1-9)


微固相萃結合電灑游離質譜法取法 (Solid Phase Microextraction-Thermal-Desorption Electrospray Ionization Mass Spectrometry,  SPME-TD-ESI/MS)      

開發一套用來快速進行藥物動力學的技術,除了可以鑑定生物體液中的微量藥物外,亦可進行藥物半定量的分析,並有效減少傳統在進行藥物動力學研究,使用液相層析質譜分析前處理時,需要耗費大量的工時及使用樣品量。


Anal. Chim. Acta (2018, 60-68)
Rapid Commun. Mass Spectrom. (2019, 34 Suppl 1, e8564)
Anal. Chim. Acta. (2020, 1107, 101-106)
     







生物醫學 (Biomedicine):

開發以基質輔助雷射脫附飛行時間式游離質譜法 (Matrix-assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry, MALDI-TOF MS) 作為臨床醫學檢驗上診斷特定疾病之生物指標 (蛋白質/胜肽、脂質、代謝物) 的分析技術,並以MALDI-TOF MS進行分子影像 (Molecular Image)研究各式生物指標在正常及疾病組織中之原位分佈。

乳癌指標 (Breast Cancer)

乳癌是因為乳房乳腺管細胞或是乳小葉細胞產生不正常分裂、繁殖而形成之惡性腫瘤。乳癌除了局部侵犯乳房組織外,更可能轉移到遠處組織,如:肺、骨骼、肝臟、腦等器官,而危害生命安全。
  糞便潛血指標 (Fecal Occult Blood, FOB)

大腸癌的腫瘤或息肉會受到糞便形成過程中的摩擦而流血,血液因而混合在糞便內排出,使得糞便表面含有肉眼看不出來的血液,透過糞便潛血檢查可以早期發現大腸癌或息肉等症狀。
  蛋白尿指標 (Albuminuria)

一般正常尿液因排泄系統構造的關係,尿中蛋白質應小於150毫克 (白蛋白每日排出量應小於30毫克),尿中蛋白質高於標準值時,可能導因於是否有腎臟或心臟方面的疾病。

Genomic Medicine, Biomarkers, and Health Sciences. (2012, 4, 3-6)
Clin. Breast Cancer (2017, 17, 373-381)
  Clin. Chim. Acta (2007, 384, 86-92)
Rapid Commun. Mass Spectrom. (2007, 21, 3311-3316)
J. Dig. Dis. (2013, 14, 474-483)
 

  Rapid Commun. Mass Spectrom. (2008, 22, 3754-3760)
Clin. Chim. Acta (2012, 413, 875-882)
Clin. Chim. Acta (2013, 420, 76-81)
 
 Ι 487911 (中華民國專利證號)

 

 
缺血性腦中風指標 (Ischemic Stroke)

當腦部血管有局部性的阻塞,使得依靠這條血管供給血液及營養的腦組織受損,造成腦細胞傷害、壞死、局部神經性機能障礙,稱之為缺血性中風,相較於出血性中風,缺血性中風發生機率較高。
  精神疾病指標 (Mental Disorders)

是一種以表現在行為、心理活動上的紊亂為主的精神症狀。其原因可能來自外在因素如家庭、社會環境,或是患者自身的生理遺傳因素、神經生化因素等內在原因相互作用所導致的心理活動、行為、及其神經系統功能紊亂,目前臨床診斷較難有具體的疾病指標判別。
  防禦素發炎指標 (Defensins)

人體免疫系統的細胞含有這些防禦素協助殺死被吞噬的細菌,具有很強的抗細菌、真菌和具外套膜病毒作用,其存於嗜中性粒細胞和幾乎所有的上皮細胞。
J. Neurol. (2009, 256, 625-631)   Rapid Commun. Mass Spectrom. (2009, 23, 589-598)
Clin. Chim. Acta (2013, 424, 175-181)
Rapid Commun. Mass Spectrom. (2016, 30, 2645-2649)
Clin Chim Acta (2017, 473, 75-81)
 
 
  Rapid Commun. Mass Spectrom. (2009, 23, 3220-3226)
Anal. Methods (2010, 2, 1934-1940)
Clin. Chim. Acta (2018, 479, 212-218)

 

 
胃幽門桿菌毒性鑑定 (Bacteria Identification) 

此菌的螺旋結構及菌上頭的鞭毛,使它能夠鑽入胃黏液而達到胃黏膜上,在黏液與黏膜之間酸度是中性的,而且此細菌本身也可以分泌尿素脢來保護自己,在胃裡面會破壞胃黏膜造成胃發炎,從急性的胃炎到慢性的胃炎它都可以一肩挑,擔任相當吃重的角色,目前已確定胃炎的重要罪魁禍首之一就是它。
  糖化血色素指標 (HbA1c)

糖化血色素是最容易反映血糖濃度的指標之一,血液中的葡萄糖濃度愈高,累積的HbA1c濃度與比率也愈高,且因為紅血球的平均壽命約為3個月,所以測定血液HbA1c的百分比,就可以估計過去120天期間內的平均血糖濃度,目前是糖尿病患者控制血糖的最佳指標。一般來說,HbA1c的正常檢測參考值約為4~6%,國際間採用之糖尿病治療準則是將HbA1c控制在7%以下。
  影像質譜法 (Mass Spectrometry Imaging , MSI)

此分析方法可利用質譜訊號來分析物質組成,更可了解特定物質在空間中的分布情形。此分析方法之優勢即是不需要使用任何標記或抗體標誌物,即可直接在組織樣品上進行分子影像觀察與研究,並了解其分子空間分布資訊,且能找出與腫瘤發生相關之生化分子。
Clin. Chim. Acta (2013, 424, 123-130)

US7,750,291B2 (美國專利證號)
I 340244 (中華民國專利證號)
   Anal. Chem. (2008, 80, 4845-4852)   Anal Methods (2010, 2, 1974-1981)
Genomic Medicine, Biomarkers, and Health Sciences (2012, 4, 3-6)
Clin. Breast Cancer (2017, 17, 373-381)


急診醫療 (Emergency Medicine):

開發熱脫附式電噴灑游離裝置結合各式高靈敏質譜儀,應用在急診真實案例上。取樣來源可自洗胃液、唾液、皮膚、殘於瓶蓋中快速分析急性中毒之毒性成份,幫助醫師做出正確的治療。

Rapid Commun. Mass Spectrom. (2015, 29, 163-170)
J. of Mass Spectrom. (2016, 51, 97-104)
Rapid Commun. Mass Spectrom.  (2016, 30, 1295-303)
Mass Spectrom. (2017, 6, S0056)
Am. J. Emerg. Med. (2018, 36, 530.e1-530.e5)
Clin Chim Acta (2018, 485, 288-297)

J. Food Drug Anal. (2019, 27, 415-427)
Anal. Chim. Acta (2019, 1066, 69-78)

Rapid Commun. Mass Spectrom. (2019, 34 Suppl 1, e8562)



食品安全 (Food Safety):

近年來因食品中毒事件時有所聞,但食品中組合成分複雜,檢測不易。本實驗室開發各種大氣質譜法結合各式質譜儀直接且快速分析目標物,像是檢測易混淆之有毒野菜、野菇中的毒素;針對各式蔬果表面的殘餘農藥;周邊物件表面的塑化劑殘留;化妝品中的防腐劑等,不僅能保障食品的食用安全,更能釐清食品食用後造成健康損害之原因來源。

Rapid Commun. Mass Spectrom. (2015, 29, 163-170)
Rapid Commun. Mass Spectrom. (2016, 30, 2315-2322)
Anal. Chim. Acta (2018, 1039, 65-73)
Int. J. Mass Spectrom. (2018, 434, 264-271)
Anal. Chim. Acta (2018, 1039, 65-73)
J. Food Drug Anal. (2019, 27, 415-427)
J. Food Drug Anal. (2019, 27, 451-459)
Anal. Methods (2019, 11, 6013-6022)


Anal. Chim. Acta
(2020, 1102, 63-71)
Anal. Chim. Acta (2020,
1105, 128-138)



刑事鑑識 (Forensic Science):
刑事鑑識一直以來都扮演著輔助案件偵查的重要角色,隨著時代的進步,越來越講究科學辦案,但傳統分析往往受限於時間與成本,導致分析的結果
動輒花上數天。本實驗開發各種大氣質譜法結合各式質譜儀,可直接對物品、不明液體、甚至皮膚上直接進行分析,具有快速、即時、高通量等優點,
為阻嚇犯罪的一大利器。
J. Mass Spectrom. (2013, 48, 1129-1135)
J. Mass Spectrom. (2014, 49, 445-51)
J. Mass Spectrom.
(2016, 51, 20-27)
Taiwan J. Forensic Medicine (2016, 8, 28-41)
J. Food Drug Anal.
(2019, 27, 451-459)


代表性著作

  1. Cho, Y. T.; Su, H.; Huang, T. L.; Chen, H. C.; Wu, W. J.; Wu, P. C.; Wu, D. C. ; Shiea, J., “Matrix-assisted Laser Desorption Ionization/time-of-flight Mass Spectrometry for Clinical Diagnosis” Clin. Chim. Acta 2013, 415, 266-275. (Invited Review Article, Citation: 42)
  2. Cheng, S.C.; Huang,  M. Z.; Shiea, J., “Thin Layer Chromatography/Mass Spectrometry”  J. Chromatogr. A, 2011, 1218, 2700-2711. (Invited Review Article, Citation: 104)
  3. Huang, M. Z.; Cho, Y. T.; Cheng, S. C.; Shiea, J., “Ambient Ionization Mass Spectrometry - A Tutorial” Anal. Chim. Acta 2011, 702, 1-15. (Invited Review Article, Citation: 257)
  4. Huang, M. Z.; Yuan, C. H.; Cheng, S. C.; Cho, Y. T.; Shiea, J., “Ambient Ionization Mass Spectrometry” Annual Review Analytical Chemistry, 2010, 3, 43-65. (Citation: 252)
  5. Shiea, J.; Huang, M. Z.; Hsu, H. J.; Lee, C. Y.; Yuan, C. H.; Beech, I.; Sunner, J., Electrospray-assisted laser desorption/ionization mass spectrometry for direct ambient analysis of solids. Rapid Commun. Mass Spectrom. 2005, 19(24), 3701-3704. (Citation: 478)
  6. Chang, D. Y.; Lee, C. C.; Shiea, J., Detecting Large Biomolecules from High-salt Solutions by Fused-droplet Electrospray Ionization Mass Spectrometry. Anal. Chem. 2002, 74(11), 2465-2469. (Citation: 131)
  7. Yuan, C. H.; Shiea, J., Sequential Electrospray Analysis Using Sharp-tip Channels Fabricated on a Plastic Chip. Anal. Chem. 2001, 73(6), 1080-1083. (Citation: 142)

專書/專刊

  1. Shiea, J.; Shimma S.; Lai, C. C., A Special Issue for 2018 Mass Spectrometry for Clinical Diagnosis. Rapid Commun. Mass Spectrom. 2020, doi: 10.1002/rcm.8702.
  2. Su, H.; Lin, J. Y.; Shiea, J., “In-situ Detection of Chemical Compounds Using Ambient Ionization Mass Spectrometry”. 2019, 57, 42-51.
  3. Shiea, J., A Special Issue for 2017 Mass Spectrometry for Clinical Diagnosis. J. Food Drug Anal. (Taiwan). 2019, 27, 375-614.
  4. Cheng, S.; Shiea, J., “Rapid Characterization of toxic compounds with Ambient Mass Spectrometric system”. SciTech Reports., 2018.
  5. Liao, P. C; Shiea, J., A Special Issue for The 13th Annual Conference of the Taiwan Society for Mass Spectrometry. Mass Spectrom. (Tokyo) . 2017, 6(2): K0010.
  6. Cheng, S.; Su, H.; Lee, R. H.; Cai, Y. D.; Lee, C. W.; Shiea, J., “Rapid Characterization of Pesticides and Drugs in Biofluids and on  Solid Samples with Thermal Desorption Electrospray Ionization Mass Spectrometry”. Taiwan I. Forensic Medicine, 2016, 8(2), 28-41.
  7. Cheng, S.; Shiea, J., “Interfacing TLC with Laser-Based Ambient Mass Spectrometry”. Planar. Chromatogr. Mass Spectrom., 2015, 110, 105-118.
  8. Su, H.; Shiea, J., “Laser-Based Molecular Imaging Mass Spectrometry ”. Instrum. Today, 2015, 203, 7-16.
  9. Huang, M. Z.; Jhang, S. S.; Shiea, J., “Electrospray Laser Desorption Ionization (ELDI) Mass Spectrometry for Molecular Imaging of Small Molecules on Tissues”. Mass Spectrom. Imaging of Small Molecules, 2015, 1203, 107-116.
  10. Cho, Y. T.; Su, H.; Wu, W. J.; Wu, D. C.; Hou, M. F.; Kuo, C. H.; Shiea, J., “Biomarker Characterization by MALDI-TOF/MS”. Adv. Clin. Chem. 2015, 69, 209-254.
  11. Huang, M. Z.; Jhang, S. S.; Chan, Y. T.; Cheng, S. C.; Cheng, C. N.; Shiea, J., “Electrospray Laser Desorption Ionization Mass Spectrometry”. Ambient Ionization Mass Spectrom., 2014, 372-388.
  12. Cheng, S.; Shiea, J., “Advanced Spectroscopic Detectors for Identification and Quantification: Mass Spectrometry”. Instrum. Thin-Layer Chromatogr., 2014, 249-278.
  13. Li, L. J.; Liao, P. C.; Shiea, J., Mass Spectrometry for Clinical Diagnosis Preface. Clin. Chim. Acta 2013, 420, 1-3.
  14. Shiea, J.; Chou, C. C.; “Mobile Ambient Mass Spectroscopy” Instrum. Today, 2012, 187, 3-9.
  15. Cheng, C. N.; Lai, J. H.; Huang, M. Z.; Oung, J. N.; Shiea, J.; “Analysis of Polar Components in Crude Oil by Ambient Mass Spectrometry" in "Crude Oil Emulsions – Composition Stability and Characterization" (ed. M. El-Sayed Abdul-Raouf). Intech, 2012, 6, 107-120.

期刊論文(1995-2020)

2020

  • Chan, H. C.; Chan, H. C.; Liang, H. J.; Lee, H. C.; Su, H.; Lee, A. S.; Shiea, J.; Tsai, W. C.; Ou, T. T.; Wu, C. C.; Chu, C. S.; Dixon, R. A.; Ke, L. Y.; Yen, J. H.; Chen, C. H., Role of Low-density Lipoprotein in Early Vascular Aging Associated With Systemic Lupus Erythematosus. Arthritis Rheumatol. 2020, doi: 10.1002/art.41213
  • Wang, C. H.; Su, H.; Chou, J. H.; Lin, J. Y.; Huang, M. Z.; Lee, C. W.; Shiea, J., Multiple Solid Phase Microextraction Combined with Ambient Mass Spectrometry for Rapid and Sensitive Detection of Trace Chemical Compounds in Aqueous Solution. Anal. Chim. Acta 2020, 1107, 101-106.
  • Su, H.; Huang, Y. J.; Huang, M. Z.; Lee, Y. T.; Chen, S. C.; Hung, C. H.; Kuo, C. H.; Wu, M. T.; Shiea, J., Using Ambient Mass Spectrometry to Explore the Origins of Phthalate Contamination in a Mass Spectrometry Laboratory. Anal. Chim. Acta 2020, 1105, 128-138.
  • Cheng, S. C.; Lee, R. H.; Jeng, J. Y.; Lee, C. W.; Shiea, J., Fast screening of trace multiresidue pesticides on fruit and vegetable surfaces using ambient ionization tandem mass spectrometry. Anal. Chim. Acta 2020, 1102, 63-71.

2019

  • Shiea, J.; Bhat, S. M.; Su, H.; Ponnusamy, V. K.; Lee, C. W.; Wang, C. H., Rapid Quantification of Acetaminophen in Plasma Using Solid Phase Microextraction Coupled with Thermal Desorption-Electrospray Ionization/Mass Spectrometry. Rapid Commun. Mass Spectrom. 2019, doi: 10.1002/rcm.8564. (Special issue)
  • Su, H.; Yeh, I. J.; Wu, Y. H.; J, Z. H.; Shiea, J.; Lee, C. W., Rapid identification of organophosphorus pesticides on contaminated skin and confirmation of adequate decontamination by ambient mass spectrometry in emergency settings. Rapid Commun. Mass Spectrom. 2019, doi: 10.1002/rcm.8562. (Special issue)
  • Su, H.; Lin Y. P.; Yang, S. C.; Kuo, C. H.; Wu, D. C.; Shiea, J.; Lee, C. W., Rapid detection of non-volatile household pesticides in drained gastric juice by ambient mass spectrometry for emergency management. Anal. Chim. Acta 2019, 1066, 69-78.
  • Su, H.; Liu, K. T.; Chen, B. H.; Lin, Y. P.; Jiang, Y. M.; Tsai, Y. H.; Chang, F. R.; Shiea, J.; Lee, C. W., Rapid Identification of Herbal Toxins Using Electrospray Laser Desorption Ionization Mass Spectrometry for Emergency Care. J. Food Drug Anal. 2019, 27, 415-427. (Cover image of special issue)
  • Cheng, S. C.; Tsai, Y. D.; Lee, C. W.; Chen, B. H.; Shiea, J., Direct and Rapid Characterization of Illicit Drugs in Adulterated Samples Using Thermal Desorption Electrospray Ionization Mass Spectrometry. J. Food Drug Anal. 2019, 27, 451-459. (Special issue)
  • Cheng, S. C.; Bhat, S. M.; Lee, C. W.; Shiea, J., Simple Interface for Scanning Chemical Compounds on Developed Thin Layer Chromatography Plates Using Electrospray Ionization Mass Spectrometry. Anal. Chim. Acta 2019, 1049, 1-9.
  • Sambandan, E.; Kathavarayan, T.; Sellappan, S.; Shiea, J.; Ponnusamy, V. K., Identification and characterization of unknown degradation impurities in beclomethasone dipropionate cream formulation using HPLC, ESI-MS and NMR. J. Pharm. Biomed. Anal. 2019, 167, 123-131.

2018

  • Lee, C. W.; Chao, Y. Y.; Shiea, J., Shen J. H., Lee, H. H.; Chen, B. H., Ambient Mass Spectrometry for Rapid Diagnosis of Psychoactive Drugs Overdose in an Unstable Patient. Am. J. Emerg. Med. 2018, 36(3), 530.e1-530.e5.
  • Wu, C. Y.; Lo, L. H.; Su, H.; Shiea, J., Detection of α-defensin in Blister Fluids as Potential Biomarkers for Bullous Pemphigoid Patients by Matrix-assisted Laser Desorption Ionization/Time-of-Flight Mass Spectrometry. Clin. Chim. Acta 2018, 479, 212-218.
  • Wang, C. H.; Su, H.; Chou, J. H.; Huang, M. Z.; Lin, H. J.; Shiea, J., Solid Phase Microextraction Combined with Thermal-desorption Electrospray Ionization Mass Spectrometry for High-throughput Pharmacokinetics Assays. Anal. Chim. Acta 2018, 60-68.
  • Lee, C. W.; Su, H.; Lee, R. H.; Lin, Y. P.; Tsai, Y. D.; Wu, D. C.; Shiea, J., Point-of-care Identification of Organophosphates in Gastric Juice by Ambient Mass Spectrometry in Emergency Settings. Clin. Chim. Acta 2018, 288-297.
  • Cheng, S. C.; Bhat, S. M.; Lee, C. W.; Shiea, J., Thin Layer Chromatography Combined with Electrospray Ionization Mass Spectrometry for Characterizing Herbal Compounds. Int. J. Mass Spectrom. 2018, 434, 264-271.
  • Su, H.; Huang, M. Z.; Chou, J. H.; Chang, T. H.; Jiang, Y. M.; Cho, Y. T.; Cheng, S. C.; Wu, M. T.; Shiea J., High-throughput Screening of Phthalate-containing Objects in the Kindergartens by Ambient Mass Spectrometry. Anal. Chim. Acta 2018, 1039, 65-73.

2017

  • Lee, C. W.; Su, H.; Cai, Y. D.; Wu, M. T.; Wu, D. C.; Shiea, J., Rapid Identification of Psychoactive Drugs in Drained Gastric Lavage Fluid and Whole Blood Specimens of Drug Overdose Patients Using Ambient Mass Spectrometry. Mass Spectrom. 2017, 6, S0056.
  • Cheng, S. C.; Chen, S. H.; Shiea, J., Desorption Flame-Induced Atmospheric Pressure Chemical Ionization Mass Spectrometry for Rapid Real-World Sample Analysis. Mass Spectrom. 2017, 6, S0065.
  • Cheng, S. C.; Shiea, C.; Huang, Y. L.; Wang, C. H.; Cho, Y. T.; Shiea, J., Laser-based Ambient Mass Spectrometry. Anal. Methods 2017, 9, 4924-4935.
  • Lin, P. D.; Wu, C. F.; Kou, H. S.; Huang, T. Y.; Shiea, J.; Wu, M. T., Removal of Diethylhexyl Phthalate from Hands by Handwashing: Evidence from Experimental N-of-1 and Crossover Designs. Sci. Rep. 2017, 7(1), 454.
  • Shiea, C.; Huang, Y. L.; Cheng, S. C.; Chen, Y. L.; Shiea, J., Determination of Elemental Composition of Metals Using Ambient Organic Mass Spectrometry. Anal. Chim. Acta 2017, 968, 50-57.
  • Satarpai, T.; Siripinyanond, A.; Su, H.; Shiea, J., Rapid Characterization of Trace Aflatoxin B1 on Groundnuts, wheat and maize by Dispersive Liquid-Liquid Microextraction Followed by Direct Electrospray Probe Tandem Mass Spectrometry. Rapid Commun. Mass Spectrom. 2017, 31, 1-9.
  • Cheng, S. C.; Bhat, S. M.; Shiea, J., Flame Atmospheric Pressure Chemical Ionization Coupled with Negative Electrospray Ionization Mass Spectrometry for Ion Molecule Reactions. J. Am. Soc. Mass Spectrom. 2017, 28(7), 1473-1481.
  • Cho, Y. T.; Su, H.; Chiang, Y. Y.; Hou, M. F.; Shiea, J.; Yuan, S. F.; Hung, W. C.; Yeh, Y. T., Fine Needle Aspiration Combined with Matrix-Assisted Laser Desorption Ionization/Time-of-Flight Mass Spectrometry to Rapidly Assist Diagnosing Breast Cancer. Clin. Breast Cancer 2017, 17(5), 373-381.
  • Huang, T. L.; Lo, L. H.; Shiea, J.; Su, H., Rapid and Simple Analysis of Disease-associated Biomarkers of Taiwanese Patients with Schizophrenia Using Matrix-assisted Laser Desorption Ionization Mass Spectrometry. Clin. Chim. Acta 2017, 473, 75–81.
  • Chang, F. K.; Shiea, J.; Tsai, H. J., Urinary Concentrations of Triclosan, Benzophenone-3, and Bisphenol A in Taiwanese Children and Adolescents. Int. J. Environ. Res. Public Health 2017, 14(12), p: E1545.

2016

  • Kao, Y. Y.; Cheng, S. C.; Cheng, C. N.; Shiea, J., Depth Profiling of Inks in Authentic and Counterfeit Banknotes by Electrospray Laser Desorption Ionization/Mass Spectrometry. J. Mass Spectrom. 2016, 51(1) , 20-7.
  • Kostyukevich, Y.; Kononikhin, A.; Popov, I.; Kukaev, E.; Shiea, J.; Nikolaev, E., Supermetallization of Peptides and Proteins with Tetravalent Metal Th(IV), Eur. J. Mass Spectrom. 2016, 22(1) , 39-42.
  • Kuo, H. K.; Chen, Y. H.; Huang, F.; Wu, Y. C.; Shiea, J.; Wu, P. C., the Upregulation of Zinc Finger Protein 670 and Prostaglandin D2 Synthase in Proliferative Vitreoretinopathy. Graefes. Arch. Clin. Exp. Ophthalmol. 2016, 254(2) , 205-213.
  • Lee, C. W.; Su, H.; Chen, P. Y.; Lin, S. J. ; Shiea, J.; Shin, S. J.; Chen, B. H., Rapid Identification of Pesticides in Human Saliva for Emergency Management by Thermal Desorption Electrospray Ionization/Mass Spectrometry. J. Mass Spectrom 2016, 51, 97-104. (Featured article)
  • Cheng, S. C.; Chen, Y. T.; Jhang, S. S.; Shiea, J., Flame-induced Atmospheric Pressure Chemical Ionization Mass Spectrometry. Rapid Commun. Mass Spectrom. 2016, 30(7), 890-6.
  • Cheng, S. C.; Wang, C. H.; Shiea, J., Formation of Metal-Adducted Analyte Ions by Flame-Induced Atmospheric Pressure Chemical Ionization Mass Spectrometry. Anal. Chem. 2016, 88(10), 5159-65.
  • Lee, C. W.; Su, H.; Wu, K. D.; Shiea, J., Wu, D. C.; Shin, S. J.; Chen, B. H. Rapid Point-of-care Identification of Mis-swallowed Oral Medications in Gastric Lavage Content by Ambient Mass Spectrometry in Emergency Room. Rapid Commun. Mass Spectrom. 2016, 30(11), 1295-1303.
  • Lo, L. H.; Shiea, J.; Huang, T. L., Rapid Detection of Alteration of Serum IgG in Patients with Schizophrenia after Risperidone Treatment by Matrix-assisted Laser Desorption Ionization/Time-of-Flight Mass Spectrometry. Rapid Commun. Mass Spectrom. 2016, 30(24), 2645-2649.

2015

  • Shiea, C.; Huang, Y. L.; Liu, D. L.; Chou, C. C.; Chou, J. H.; Chen, P. Y.; Shiea, J.; Huang, M. Z., Rapid Screening of Residual Pesticides on Fruits and Vegetables Using Thermal Desorption Electrospray Ionization Mass Spectrometry. Rapid Commun. Mass Spectrom. 2015, 29, 163–170.
  • Cheng, S. C.; Chang, S. S.; Huang, M. Z.; Shiea, J., Simultaneously Detecting Polar and Nonpolar Compounds by Ambient ESI/APCI Mass Spectrometry, Anal. Chem. 2015, 87, 1743-1748.
  • Kuo, F. C.; Su, S. W.; Wu, C. F.; Huang, M. C.; Shiea, J.; Chen, B. H.; Chen, Y. L.; Wu, M. T., Relationship of Urinary Phthalate Metabolites with Serum Thyroid Hormones in Pregnant Women and Their Newborns: a Prospective Birth Cohort in Taiwan. PLoS One 2015, 10(6), e0123884.

2014

  • Cho, Y. T.; Huang, M. Z.; Wu, S. Y.; Hou, M. F.; Li, J. J.; Shiea, J., Using Electrospray Laser Desorption Ionization Mass Spectrometry to Rapidly Examine the Integrity of Proteins Stored in Various Solutions. Anal. Bioanal. Chem. 2014, 406(2), 577-586.
  • Kao, Y. Y.; Cheng, S. C.; Cheng, C. N.; Shiea, J.; Ho, H. O., Detection of Trace Ink Compounds in Erased Handwritings Using Electrospray-assisted Laser Desorption Ionization Mass Spectrometry. J. Mass Spectrom. 2014, 49(6), 445-51.
  • Wu, C. F.; Liu, C. C.; Chou, Y. H.; Shiea, J.; Shen, J. T.; Wang, S. S.; Wu, M. T., Increased Detection Rate of Melamine-containing Calcium Urolithiasis by Using Matrix-assisted Laser Desorption/ionization Time-of-flight Mass Spectrometry Technique in Clinical Practice (vol 431C, pg294, 2014). Clin. Chim. Acta 2014, 435, 62-63.
  • Hsu, F. J.; Liu, T. L.; Laskar, A. H.; Shiea, J.; Huang, M. Z., Gravitational Sampling Electrospray Ionization Mass Spectrometry for Real‐time Reaction Monitoring. Rapid Commun. Mass Spectrom. 2014, 28(18), 1979-1986.

2013

  • Wu, M. T.; Wu, C. F.; Chen, B. H.; Chen, E. K.; Chen, Y. L.; Shiea, J.; Lee, W. T.; Chao, M. C.; Wu, J. R., Intake of Phthalate-tainted Foods Alters Thyroid Functions in Taiwanese Children. PloS one 2013, 8(1), e55005.
  • Cho, Y. T.; Su, H.; Huang, T. L.; Chen, H. C.; Wu, W. J.; Wu, P. C.; Wu, D. C.; Shiea, J., Matrix-assisted Laser Desorption Ionization/Time-of-flight Mass Spectrometry for Clinical Diagnosis. Clin. Chim. Acta 2013, 415, 266-275.
  • Kononikhin, A.; Huang, M. Z.; Popov, I.; Kostyukevich, Y.; Kukaev, E.; Boldyrev, A.; Spasskiy, A.; Leypunskiy, I.; Shiea, J.; Nikolaev, E., Signal Enhancement in Electrospray Laser Desorption/Ionization Mass Spectrometry by Using a Black Oxide-coated Metal Target and a Relatively Low Laser Fluence. Eur. J. Mass Spectrom. 2013, 19(4), 247-252.
  • Chiu, K. H.; Hsieh, F. M.; Chen, Y. Y.; Huang, H. W.; Shiea, J.; Mok, H. K., Parvalbumin Characteristics in the Sonic Muscle of a Freshwater Ornamental Grunting Toadfish (Allenbatrachus grunniens). Fish Physiol. Biochem. 2013, 39(2), 107-119.
  • Cho, Y. T.; Hu, J. L.; Shiea, J.; Chen, C. W.; Su, H.; Chen, H. C.; Wu, W. J.; Wu, D. C., Diagnosis of Albuminuria by Tryptic Digestion and Matrix-assisted Laser Desorption Ionization/Time-of-flight Mass Spectrometry.Clin. Chim. Acta 2013, 420, 76-81.
  • Lin, Y. T.; Tsai, M. T.; Chen, Y. L.; Cheng, C. M.; Hung, C. C.; Wu, C. F.; Liu, C. C.; Hsieh, T. J.; Shiea, J.; Chen, B. H.; Wu, M. T., Can Melamine Levels in 1-spot Overnight Urine Specimens Predict the Total Previous 24-hour Melamine Excretion Level in School children. Clin. Chim. Acta 2013, 420, 128-133.
  • Cho, Y. T.; Kuo, C. H.; Wang, S. S. W.; Chen, Y. S.; Weng, B. C.; Lee, Y. C.; Cheng, C. N.; Shiea, J.; Wu, D. C., Differentiation of Virulence of Helicobacter Pylori by Matrix-assisted Laser Desorption/Ionization Mass Spectrometry and Multivariate Analyses. Clin. Chim. Acta 2013, 424, 123-130.
  • Ou, C. H.; Kuo, F. C.; Hsu, W. H.; Lu, C. Y.; Yu, F. J.; Kuo, C. H.; Wang, J. Y.; Wu, M. T.; Shiea, J.; Wu, D. C.; Hu, H. M., Comparison of the Performance of Guaiac-based and Two Immunochemical Fecal Occult Blood Tests for Identifying Advanced Colorectal Neoplasia in Taiwan. J. Dig. Dis. 2013, 14(9), 474-83.
  • Huang, M. Z.; Zhou, C. C.; Liu, D. L.; Jhang, S. S.; Cheng, S. C.; Shiea, J., Rapid Characterization of Chemical Compounds in Liquid and Solid States Using Thermal Desorption Electrospray Ionization Mass Spectrometry. Anal. Chem. 2013, 85(19), 8956-8963.
  • Huang, T. L.; Cho, Y. T.; Su, H.; Shiea. J., Principle Component Analysis Combined with Matrix-assisted Laser Desorption Ionization Mass Spectrometry for Rapid Diagnosing the Sera of Patients with Major Depression.Clin. Chim. Acta 2013, 424, 175-181.
  • Kao, Y. Y.; Cheng, C. N.; Cheng, S. C.; Ho, H. O.; Shiea, J., Distinguishing Authentic and Counterfeit Banknotes by Surface Chemical Composition Determined Using Electrospray Laser Desorption Ionization Mass Spectrometry. J. Mass Spectrom. 2013, 48(11), 1129-1135.
  • Wu, C. F.; Chen, B. H.; Shiea, J. T.; Chen, E. K.; Liu, C. K.; Chao, M. C.; Ho, C. K.; Wu, J. R.; Wu, M. T., Temporal Changes of Urinary Oxidative Metabolites of Di(2-ethylhexyl)phthalate After the 2011 Phthalate Incident in Taiwanese Children: Findings of a Six Month Follow-Up. Environ. Sci. Technol. 2013, 47(23), 13754-13762.

2012

  • Chuang, C. H.; Chuang, K. H.; Wang, H. E.; Roffler, S. R.; Shiea, J. T.; Tzou, S. C.; Cheng, T. C.; Kao, C. H.; Wu, S. Y.; Tseng, W. L.; Cheng, C. M.; Hou, M. F.; Wang, J. M.; Cheng, T. L., In Vivo Positron Emission Tomography Imaging of Protease Activity by Generation of a Hydrophobic Product from a Noninhibitory Protease Substrate. Clin. Cancer Res. 2012, 18(1), 238-47.
  • Cho, Y. T.; Chen, Y. S.; Hu, J. L.; Shiea, J.; Yeh, S. M.; Chen, H. C.; Lee, Y. C.; Wu, D. C., The Study of Interferences for Diagnosing Albuminuria by Matrix-assisted Laser Desorption Ionization/Time-of-flight Mass Spectrometry. Clin. Chim. Acta 2012, 413(9-10), 875-882.
  • Y. T. Cho , Y. Y. Chiang, J. Shiea, M. F. Hou, Combining MALDI-TOF and Molecular Imaging with Principal Component Analysis for Biomarker Discovery and Clinical Diagnosis of Cancer. Genomic Medicine, Biomarkers, and Health Sciences. 2012, 4, 3-6.
  • Kuo, H. K.; Chen, Y. H.; Wu, P. C.; Wu, Y. C.; Huang, F.; Kuo, C. W.; Lo, L. H.; Shiea, J., Attenuated Glial Reaction in Experimental Proliferative Vitreoretinopathy Treated with Liposomal Doxorubicin. Invest. Ophthalmol. Visual Sci. 2012, 53(6), 3167-74.
  • Cheng, S. C.; Huang, M. Z.; Wu, L. C.; Chou, C. C.; Cheng, C. N.; Jhang, S. S.; Shiea, J., Building Blocks for the Development of an Interface for High-Throughput Thin Layer Chromatography/Ambient Mass Spectrometric Analysis: A Green Methodology. Anal. Chem. 2012, 84(14), 5864-5868.
  • Huang, M. Z.; Cheng, S. C.; Jhang, S. S.; Chou, C. C.; Cheng, C. N.; Shiea, J.; Popov, I. A.; Nikolaev, E. N., Ambient Molecular Imaging of Dry Fungus Surface by Electrospray Laser Desorption Ionization Mass Spectrometry. Int. J. Mass Spectrom. 2012, 325, 172-182.
  • Chin, K. H.; Lin, C. R.; Huang, H. W.; Shiea, J.; Liu, L. L., Toxic Effects of Two Brominated Flame Retardants BDE-47 and BDE-183 on the Survival and Protein Expression of the Tubificid Monopylephorus Limosus. Ecotoxicol. Environ. Saf. 2012, 84, 46-53.
  • Liu, C. C.; Wu, C. F.; Shiea, J.; Cho, Y. T.; Hsieh, T. J.; Chou, Y. H.; Chen, B. H.; Huang, S. P.; Wu, W. J.; Shen, J. T.; Chang, M. Y.; Huang, C. H.; Chang, A. W.; Wu, M. T., Detection of Melamine in a Human Renal Uric Acid Stone by Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry (MALDI-TOF MS). Clin. Chim. Acta 2012, 413(19-20), 1689-1695.

2011

  • Chien, C. Y.; Wu, C. F.; Liu, C. C.; Chen, B. H.; Huang, S. P.; Chou, Y. H.; Chang, A. W.; Lee, H. H.; Pan, C. H.; Wu, W. J.; Shen, J. T.; Chang, M. Y.; Huang, C. H.; Shiea, J.; Hsieh, T. J.; Wu, M. T., High Melamine Migration in Daily-use Melamine-made Tableware. J. Hazard. Mater. 2011, 188(1-3), 350-356.
  • Cheng, S. C.; Huang, M. Z.; Shiea, J., Thin Layer Chromatography/Mass Spectrometry. J. Chromatogr. A. 2011, 1218(19), 2700-2711.
  • Kuo, S. M.; Yang, C. C.; Shiea, J.; Lin, C. H., A Post-bonding-free Fabrication of Integrated Microfluidic Devices for Mass Spectrometry Applications. Sensor Actuat. B-Chem. 2011, 156(1), 156-161.
  • Huang, M. Z.; Cheng, S. C.; Cho, Y. T.; Shiea, J., Ambient Ionization Mass Spectrometry: A Tutorial. Anal. Chim. Acta 2011, 702(1), 1-15.
  • Lin, Y. C.; Chiu, K. H.; Shiea, J.; Huang, H. W.; Mok, H. K., Seasonal Changes in Atrophy-associated Proteins of the Sonic Muscle in the Big-snout Croaker, Johnius Macrorhynus (Pisces, Sciaenidae), Identified by Using a Proteomic Approach. Fish Physiol. Biochem. 2011, 37(4), 977-991.

2010

  • Cheng, S. C.; Lin, Y. S.; Huang, M. Z.; Shiea, J., Applications of Electrospray Laser Desorption Ionization Mass Spectrometry for Document Examination. Rapid Commun. Mass Spectrom. 2010, 24(2), 203-208.
  • Hung, W. C.; Tseng, W. L.; Shiea, J.; Chang, H. C., Skp2 Overexpression Increases the Expression of MMP-2 and MMP-9 and Invasion of Lung Cancer Cells. Cancer Lett. 2010, 288(2), 156-161.
  • Huang, M. Z.; Jhang, S. S.; Cheng, C. N.; Cheng, S. C.; Shiea, J., Effects of Matrix, Electrospray Solution, and Laser Light on the Desorption and Ionization Mechanisms in Electrospray-assisted Laser Desorption Ionization Mass Spectrometry. Analyst. 2010, 135(4), 759-766.
  • Huang, M. Z.; Yuan, C. H.; Cheng, S. C.; Cho, Y. T.; Shiea, J., Ambient Ionization Mass Spectrometry. Annu. Rev. Anal. Chem. 2010, 3, 43-65.
  • Chuang, K. H.; Tzou, S. C.; Cheng, T. C.; Kao, C. H.; Tseng, W. L.; Shiea, J.; Liao, K. W.; Wang, Y. M.; Chang, Y. C.; Huang, B. J.; Wu, C. J.; Chu, P. Y.; Roffler, S. R.; Cheng, T. L., Measurement of Poly(ethylene glycol) by Cell-based Anti-poly(ethylene glycol) ELISA. Anal. Chem. 2010, 82(6), 2355-2362.
  • Chuang, K. H.; Wang, H. E.; Chen, F. M.; Tzou, S. C.; Cheng, C. M.; Chang, Y. C.; Tseng, W. L.; Shiea, J.; Lin, S. R.; Wang, J. Y.; Chen, B. M.; Roffler, S. R.; Cheng, T. L., Endocytosis of PEGylated Agents Enhances Cancer Imaging and Anticancer Efficacy. Mol. Cancer Ther. 2010, 9(6), 1903-12.
  • Wang, H. Y.; Lo, L. H.; Tyan, Y. C.; Chen, H. C.; Yang, M. H.; Chen, T. Y.; Wang, H. Y. J.; Shiea, J., The Study of the Distribution of Melamine in Rat Renal Tissues by Imaging Mass Spectrometry. Anal. Methods-Uk. 2010, 2(12), 1974-1981.
  • Lo, L. H.; Wu, P. C.; Wu, Y. C.; Shiea, J., Characterization of Human Neutrophil Peptides (alpha-Defensins) in the Tears of Dry Eye Patients. Anal. Methods-Uk. 2010, 2(12), 1934-1940.

2009

  • Cheng, C. M.; Chu, P. Y.; Chuang, K. H.; Roffler, S. R.; Kao, C. H.; Tseng, W. L.; Shiea, J.; Chang, W. D.; Su, Y. C.; Chen, B. M.; Wang, Y. M.; Cheng, T. L., Hapten-derivatized Nanoparticle Targeting and Imaging of Gene Expression by Multimodality Imaging Systems. Cancer Gene Ther. 2009, 16(1), 83-90.
  • Lo, L. H.; Huang, T. L.; Shiea, J., Acid Hydrolysis Followed by Matrix-assisted Laser Desorption/Ionization Mass Spectrometry for the Rapid Diagnosis of Serum Protein Biomarkers in Patients with Major Depression. Rapid Commun. Mass Spectrom. 2009, 23(5), 589-598.
  • Chen, Y. H.; Wu, P. C.; Shiea, J.; Lo, L. H.; Wu, Y. C.; Kuo, H. K., Evaluation of the Sterility, Stability, and Efficacy of Bevacizumab Stored in Multiple-dose Vials for 6 Months. J. Ocul. Pharmacol. Th. 2009, 25(1), 65-69.
  • Cheng, S. C.; Cheng, T. L.; Chang, H. C.; Shiea, J., Using Laser-Induced Acoustic Desorption/Electrospray Ionization Mass Spectrometry To Characterize Small Organic and Large Biological Compounds in the Solid State and in Solution under Ambient Conditions. Anal. Chem. 2009, 81(3), 868-874.
  • Huang, P. Y.; Lo, L. H.; Chen, Y. C.; Lin, R. T.; Shiea, J.; Liu, C. K., Serum Free Hemoglobin as a Novel Potential Biomarker for Acute Ischemic Stroke. J. Neurol. 2009, 256(4), 625-631.
  • Tzou, S. C.; Roffler, S.; Chuang, K. H.; Yeh, H. P.; Kao, C. H.; Su, Y. C.; Cheng, C. M.; Tseng, W. L.; Shiea, J.; Harm, I. H.; Cheng, K. W.; Chen, B. M.; Hwang, J. J.; Cheng, T. L.; Wang, H. E., Micro-PET Imaging of beta-Glucuronidase Activity by the Hydrophobic Conversion of a Glucuronide Probe. Radiology 2009, 252(3), 754-62.
  • Tyan, Y. C.; Yang, M. H.; Jong, S. B.; Wang, C. K.; Shiea, J., Melamine Contamination. Anal. Bioanal. Chem. 2009, 395(3), 729-735.
  • Yang, M. H.; Lo, L. H.; Chen, Y. H.; Shiea, J.; Wu, P. C.; Tyan, Y. C.; Jong, Y. J., Study of Human Neutrophil Peptides in Saliva by Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry. Rapid Commun. Mass Spectrom. 2009, 23(19), 3220-6.
  • Cheng, S. C.; Huang, M. Z.; Shiea, J., Thin-Layer Chromatography/Laser-induced Acoustic Desorption/Electrospray Ionization Mass Spectrometry. Anal. Chem. 2009, 81(22), 9274-9281.

2008

  • Shiea, J.; Yuan, C. H.; Huang, M. Z.; Cheng, S. C.; Ma, Y. L.; Tseng, W. L.; Chang, H. C.; Hung, W. C., Detection of Native Protein Ions in Aqueous Solution under Ambient Conditions by Electrospray Laser Desorption/Ionization Mass Spectrometry. Anal. Chem. 2008, 80(13), 4845-4852.
  • Cheng, C. M.; Lu, Y. L.; Chuang, K. H.; Hung, W. C.; Shiea, J.; Su, Y. C.; Kao, C. H.; Chen, B. M.; Roffler, S.; Cheng, T. L., Tumor-targeting Prodrug-activating Bacteria for Cancer Therapy. Cancer Gene Ther. 2008, 15(6), 393-401.
  • Peng, I. X.; Loo, R. R. O.; Shiea, J.; Loo, J. A., Reactive-electrospray-assisted Laser Desorption/Ionization for Characterization of Peptides and Proteins. Anal. Chem. 2008, 80(18), 6995-7003.
  • Chuang, P. H.; Hsieh, P. W.; Yang, Y. L.; Hua, K. F.; Chang, F. R.; Shiea, J.; Wu, S. H.; Wu, Y. C., Cyclopeptides with Anti-inflammatory Activity from Seeds of Annona Montana. J. Nat. Prod. 2008, 71(8), 1365-1370.
  • Cheng, C. Y.; Yuan, C. H.; Cheng, S. C.; Huang, M. Z.; Chang, H. C.; Cheng, T. L.; Yeh, C. S.; Shiea, J., Electrospray-assisted Laser Desorption/Ionization Mass Spectrometry for Continuously Monitoring the States of Ongoing Chemical Reactions in Organic or Aqueous Solution under Ambient Conditions. Anal. Chem. 2008, 80(20), 7699-7705.
  • Wu, I. C.; Wu, M. T.; Chen, Y. K.; Hsu, M. C.; Chou, S. H.; Lee, C. H.; Shiea, J. T.; Wu, I. L.; Huang, C. T.; Wu, D. C., Apoptotic Effect of Helicobacter Pylori on Oesophageal Squamous-cell Carcinoma Cells in Vitro. Eur. J. Clin. Invest. 2008, 38(10), 760-765.
  • Shiea, J.; Cho, Y. T.; Lin, Y. H.; Chang, C. W.; Lo, L. H.; Lee, Y. C.; Ke, H. L.; Wu, W. J.; Wu, D. C., Using Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry to Rapidly Screen for Albuminuria. Rapid Commun. Mass Spectrom. 2008, 22(23), 3754-3760.

2007

  • Pan, C. T.; Shiea, J.; Shen, S. C., Fabrication of an Integrated Piezo-electric Micro-nebulizer for Biochemical Sample Analysis. J. Micromech. Microeng. 2007, 17(3), 659-669.
  • Shiea, J.; Sunner, J., The Encyclopedia of Mass Spectrom. (ed. M. Gross). Fused-Droplet Electrospray Ionization and Electrospray Laser Desorption Ionization. Elsevier,2007, 6, 528-532.
  • Lee, K. H.; Chen, S. J.; Jeng, J. Y.; Cheng, Y. C.; Shiea, J. T.; Chang, H. T., Fluorescence and Interactions with Thiol Compounds of Nile Red-adsorbed Gold Nanoparticles. J. Colloid. Interf. Sci. 2007, 307(2), 340-348.
  • Hsu, H. J.; Oung, J. N.; Kuo, T. L.; Wu, S. H.; Shiea, J., Using Electrospray-assisted Pyrolysis Ionization/Mass Spectrometry for the Rapid Characterization of Trace Polar Components in Crude Oil, Amber, Humic Substances, and Rubber Samples. Rapid Commun. Mass Spectrom. 2007, 21(3), 375-384.
  • Peng, I. X.; Shiea, J.; Loo, R. R. O.; Loo, J. A., Electrospray-assisted Laser Desorption/Ionization and Tandem Mass Spectrometry of Peptides and Proteins. Rapid Commun. Mass Spectrom. 2007, 21(16), 2541-2546.
  • Jeng, J. Y.; Lin, M. F.; Cheng, F. Y.; Yeh, C. S.; Shiea, J. T., Using High-concentration Trypsin-immobilized Magnetic Nanoparticles for Rapid in Situ Protein Digestion at Elevated Temperature. Rapid Commun. Mass Spectrom. 2007, 21(18), 3060-3068.
  • Wu, C. I.; Tsai, C. C.; Lu, C. C.; Wu, P. C.; Wu, D. C.; Lin, S. Y.; Shiea, J., Diagnosis of Occult Blood in Human Feces Using Matrix-assisted Laser Desorption Ionization/Time-of-flight Mass Spectrometry. Clin. Chim. Acta 2007, 384(1-2), 86-92.
  • Lin, S. Y.; Shih, S. H.; Wu, D. C.; Lee, Y. C.; Wu, C. I.; Lo, L. H.; Shiea, J., Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry for the Detection of Hemoglobins as the Protein Biomarkers for Fecal Occult Blood. Rapid Commun. Mass Spectrom. 2007, 21(20), 3311-3316.
  • Lin, S. Y.; Huang, M. Z.; Chang, H. C.; Shiea, J., Using Electrospray-assisted Laser Desorption/Ionization Mass Spectrometry to Characterize Organic Compounds Separated on Thin-layer Chromatography Plates. Anal. Chem. 2007, 79(22), 8789-8795.

2006

  • Huang, M. Z.; Hsu, H. J.; Lee, L. Y.; Jeng, J. Y.; Shiea, J. T., Direct Protein Detection from Biological Media Through Electrospray-assisted Laser Desorption Ionization/Mass Spectrometry. J. Proteome Res. 2006, 5(5), 1107-1116.
  • Chiu, Y. K.; Huang, C. C.; Jeng, J.; Shiea, J.; Chen, W. J., Foreign Body Granuloma Caused by Monosodium Glutamate After BCG Vaccination. J. Am. Acad. Dermatol. 2006, 55(2), S1-S5.

2005

  • Chen, C. N.; Jones, P. R.; Shiea, J., Detection of Preformed Macrocyclic Nickel Complex Ions Using Two-step Electrospray Ionization Mass Spectrometry. J. Mass Spectrom. 2005, 40(3), 413-415.
  • Shieh, I. F.; Lee, C. Y.; Shiea, J., Eliminating the Interferences from TRIS Buffer and SDS in Protein Analysis by Fused-droplet Electrospray Ionization Mass Spectrometry. J. Proteome Res. 2005, 4(2), 606-612.
  • H. J. Hsu, T. L. Kuo, S. H. Wu, J. N. Oung, J. Shiea, Characterization of Synthetic Polymers by Electrospray-assisted Pyrolysis Ionization-mass Spectrometry. Anal. Chem. 2005, 77, 7744-7749.
  • Jeng, J. Y.; Lin, C. H.; Shiea, J., Electrospray from Nanostructured Tungsten Oxide Surfaces with Ultralow Sample Volume. Anal. Chem. 2005, 77(24), 8170-8173.
  • Shiea, J.; Huang, M. Z.; Su, H. J.; Lee, C. Y.; Yuan, C. H.; Beech, I.; Sunner, J., Electrospray-assisted Laser Desorption/Ionization Mass Spectrometry for Direct Ambient Analysis of Solids. Rapid Commun. Mass Spectrom. 2005, 19(24), 3701-3704.
  • Hsu, J. L.; Huang, S. Y.; Shiea, J. T.; Huang, W. Y.; Chen, S. H., Beyond Quantitative Proteomics: Signal Enhancement of the a(1) Ion as a Mass Tag for Peptide Sequencing Using Dimethyl Labeling. J. Proteome Res. 2005, 4(1), 101-108.

2003

  • Hsu, F. L.; Chen, C. H.; Yuan, C. H.; Shiea, J., Interfaces to Connect Thin-layer Chromatography with Electrospray Ionization Mass Spectrometry. Anal. Chem. 2003, 75(10), 2493-2498.
  • Jeng, J. Y.; Shiea, J., Electrospray Ionization from a Droplet Deposited on a Surface-modified Glass Rod. Rapid Commun. Mass Spectrom. 2003, 17(15), 1709-1713.
  • Shiea, J. T.; Huang, J. P.; Teng, C. F.; Jeng, J. Y.; Wang, L. Y.; Chiang, L. Y., Use of a Water-soluble Fullerene Derivative as Precipitating Reagent and Matrix-assisted Laser Desorption/Ionization Matrix to Selectively Detect Charged Species in Aqueous Solutions. Anal. Chem. 2003, 75(14), 3587-3595.

2002

  • Lee, C. C.; Chang, D. Y.; Jeng, J.; Shiea, J., Generating Multiply Charged Protein Ions via Two-step Electrospray Ionization Mass Spectrometry. J. Mass Spectrom. 2002, 37(1), 115-7.
  • Chang, D. Y.; Lee, C. C.; Shiea, J., Detecting Large Biomolecules from High-salt Solutions by Fused-droplet Electrospray Ionization Mass Spectrometry. Anal. Chem. 2002, 74(11), 2465-2469.
  • Hsiu, S. I.; Huang, J. F.; Sun, I. W.; Yuan, C. H.; Shiea, J., Lewis Acidity Dependency of the Electrochemical Window of Zinc Chloride-1-ethyl-3-methylimidazolium Chloride Ionic Liquids. Electrochim Acta 2002, 47(27), 4367-4372.

2001

  • Yuan, C. H.; Shiea, J., Sequential Electrospray Analysis Using Sharp-tip Channels Fabricated on a Plastic Chip. Anal. Chem. 2001, 73(6), 1080-1083.
  • Leu, C. M.; Shu, C. F.; Teng, C. F.; Shiea, J., Dendritic Poly(ether-imide)s: Synthesis, Characterization, and Modification. Polymer 2001, 42(6), 2339-2348.
  • Wang, L. Y.; Chu, C. C.; Shiea, J.; Chiang, L. Y., Synthesis and Characterization of Star-brabched Oilgoanilines. Synthetic Metals. 2001, 119, 97-98.
  • Chen, H. J. C.; Chen, Y. M.; Wang, T. F.; Wang, K. S.; Shiea, J., 8-nitroxanthine, an Adduct Derived from 2 '-deoxyguanosine or DNA Reaction with Nitryl Chloride. Chem. Res. Toxicol. 2001, 14(5), 536-546.
  • Shiea, J.; Chang, D. Y.; Lin, C. H.; Jiang, S. J., Generating Multiply Charged Protein Ions by Ultrasonic Nebulization/Multiple Channel-electrospray Ionization Mass Spectrometry. Anal. Chem. 2001, 73(20), 4983-4987.

2000

  • Chen, Y. C.; Shiea, J.; Sunner, J., Rapid Determination of Trace Nitrophenolic Organics in Water by Combining Solid-phase Extraction with Surface-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry. Rapid Commun. Mass Spectrom. 2000, 14(2), 86-90.
  • Hong, C. M.; Tsai, F. C.; Shiea, J., A Multiple Channel Electrospray Source Used to Detect Highly Reactive Ketenes from a Flow Pyrolyzer. Anal. Chem. 2000, 72(6), 1175-1178.
  • Leu, C. M.; Chang, Y. T.; Shu, C. F.; Teng, C. F.; Shiea, J., Synthesis and Characterization of Dendritic Poly(ether imide)s. Macromolecules 2000, 33(8), 2855-2861.
  • Kuo, C. P.; Yuan, C. H.; Shiea, J., Generation of Electrospray from a Solution Predeposited on Optical Fibers Coiled with a Platinum Wire. J. Am. Soc. Mass Spectrom. 2000, 11(5), 464-467.

1999

  • Hong, C. M.; Lee, C. T.; Lee, Y. M.; Kuo, C. P.; Yuan, C. H.; Shiea, J., Generating Electrospray from Solutions Predeposited on a Copper Wire. Rapid Commun. Mass Spectrom. 1999, 13(1), 21-25.
  • Huang, M. W.; Chei, H. L.; Huang, J. P.; Shiea, J., Application of Organic Solvents as Matrixes to Detect Air-sensitive and Less Polar Compounds Using Low-temperature Secondary Ion Mass Spectrometry. Anal. Chem. 1999, 71(14), 2901-2907.
  • Huang, J. P.; Wang, L. Y.; Chiang, L. Y.; Shiea, J., Polyfunctional Fullerene Derivatives as UV-MALDI Matrices to Detect Peptides and Proteins. Fullerene Sci. Techn. 1999, 7(4), 541-550.
  • Huang, J. P.; Yuan, C. H.; Shiea, J.; Chen, Y. C., Rapid Screening for Diuretic Doping Agents in Urine by C(60)-assisted Laser-desorption-ionization-time-of-flight Mass Spectrometry. J. Anal. Toxicol. 1999, 23(5), 337-342.
  • Kuo, C. P.; Shiea, J., Applications of Direct Electrospray Probe/Mass Spectrometry to Detect Proteins in Liquid and Solid Phase and to Couple to Solid Phase Mircoextraction to Detect Trace Surfactants in Water. Anal. Chem. 1999, 71, 4413-4417.

1998

  • Chen, B. H.; Huang, J. P.; Wang, L. Y.; Shiea, J.; Chen, T. L.; Chiang, L. Y., Synthesis of Octadecahydroxylated C-70. Synthetic Commun. 1998, 28(19), 3515-3525.
  • Chen, B. H.; Huang, J. P.; Wang, L. Y.; Shiea, J.; Chen, T. L.; Chiang, L. Y., Facile Sulfation of C-60 Using P2O5 as an Oxidation Promoter. J. Chem. Soc. Perkin Trans. 1 1998, (7), 1171-1173.
  • Chi, Y.; Bhonsle, J. B.; Canteenwala, T.; Huang, J. P.; Shiea, J.; Chen, B. J.; Chiang, L. Y., Novel Water-soluble Hexa(sulfobutyl)fullerenes as Potent Free Radical Scavengers. Chem. Lett. 1998, (5), 465-466.
  • Lee, C. Y.; Shiea, J., Gas Chromatography Connected to Multiple Channel Electrospray Ionization Mass Spectrometry for the Detection of Volatile Organic Compounds. Anal. Chem. 1998, 70(13), 2757-2761.
  • Chen, Y. C.; Shiea, J.; Sunner, J., Thin-layer Chromatography Mass Spectrometry Using Activated Carbon, Surface-assisted Laser Desorption/Ionization. J. Chromatogr. A. 1998, 826(1), 77-86.
  • Wang, C. H.; Huang, M. W.; Lee, C.Y.; Huang, J. B.; Shiea, J, Dection of Thermally Unstable Intermediate in the Wittig Reaction Using Low Temperature Liquid Secondary Ion and Electrospray Ionization Mass Spectrometry. J. Am. Soc. Mass Spectrom. 1998, 9, 1168-1174.
  • Wang, W. S.; Tseng, P. W.; Chou, C. H.; Shiea, J., Detection of Reactive 1,2,3-hexatriene-5-one Monomer by Low-temperature Atmospheric Pressure Ionization Mass Spectrometry. Rapid Commun. Mass Spectrom. 1998, 12(14), 931-934.

1997

  • Shia, J. T.; Wang, C. H., Applications of Multiple Channel Electrospray Ionization Sources for Biological Sample Analysis. J. Mass Spectrom. 1997, 32(2), 247-250.

1996

  • J. Shiea, W. S. Wang, C. H. Wang, P. S. Chen, C. H. Chou.; Analysis of a Reactive Dimethylenedihydrothiophene in Methylene-chloride by Low-temperature Atmospheric-pressure Ionization Mass-spectrometry. Anal. Chem. 1996, 68, 1062-1066.
  • Chen, Y. C.; Chei, H. L.; Shiea, J., Analysis of Gaseous Ammonia, Volatile Primary Amines and Quaternary Ammonium Salts at Subambient Temperature by Liquid Secondary Ion Mass Spectrometry. J. Mass Spectrom. 1996, 31(5), 464-471.

1995

  • Chen, Y. C.; Shiea, J., Selective Enhancement of Ion Signal of Charged Surfactant in Fast-atom-bombardment Mass-spectrometry. J. Mass Spectrom. 1995, 30(10), 1435-1440.

專利

Category TITLE OF THE INVENTION COUNTRY PATENT NUMBER PATENT PERIOD
發明專利

ELECTROSPRAY-ASSISTED LASER DESORPTION IONIZATION DEVICE, MASS SPECTROMETER, AND METHOD FOR MASS SPECTROMETRY 經電噴灑輔助之雷射脫附游離裝置、質譜儀,以及質譜分析方法

美國 US 7696475 B2 2007.08-2025.08
發明專利 DETECTION OF NH3 FOR THE PRESENCE OF HELICOBACTER 美國 US 7750291 B2 2008.08-2026.08
發明專利

MASS SPECTROMETRIC IMAGING METHOD UNDER AMBIENT CONDITIONS USING ELECTROSPRAY-ASSISTED LASER DESORPTION 在常壓條件下以ELDI進行分子造影方法

美國 US 7,687,772 B2
2007.08-2025.08
發明專利 MASS SPECTROSCOPIC REACTION-MONITORING METHOD 美國 US 7718958 B2 2007.08-2025.08
發明專利 經電噴灑輔助之雷射脫附游離裝置、質譜儀,以及質譜分析方法 中華民國 I 271771 2007.01-2026.01
發明專利 用於偵測一固態樣品中之待測物的質譜分析方法 中華民國 I 334027 2010.12-2027.04
發明專利 用以監控在一液態樣品中之化學反應的質譜方法 中華民國 I 336429 2011.01-2027.04
發明專利 整合式微電灑晶片及其製造方法 中華民國 275791 2005.03-2025.03
發明專利 RF-DART MASS SPECTROMETRIC METHOD AND MASS SPECTROMETER FOR ANALYZINGAVAPORIZED SAMPLE 美國 US7,667,197 B2 2007.08-2027.02
發明專利

Mass Spectrometric Method and Mass Spectrometer for Analyzing a Vaporized Sample 用以分析一流液體中所含之氣態分析物的質譜儀,及用以檢驗氨氣的質譜


美國

US7,750,291 B2

2011.04-2027.08

發明專利 用以分析一流體中所含之氣態分析物的質譜儀,及用以檢驗氨氣的質譜分析方法 中華民國 I 340244 2011.04-2027.08
發明專利 旋轉式電噴灑游離裝置、質譜儀,以及質譜分析方法 美國 US 8,258,469 B2 2012.09-2031.03
發明專利 旋轉式電噴灑游離裝置、質譜儀,以及質譜分析方法 日本 5193329

2011.03-2031.03

發明專利 電噴灑游離裝置、質譜儀,以及質譜分析方法 中華民國 430322 2014.03-2031.03
發明專利 旋轉式脫附電噴灑游離裝置及質譜系統 中華民國 I 435367 2014.04-2030.09
發明專利 旋轉式大氣壓力化學游離裝置 中華民國 I 435365 2014.04-2030.10
發明專利 經電噴灑輔助之熱脫附游離裝置、質譜儀、以及質譜分析方法 中華民國 I 435366 2014.04-2031.05
發明專利 以直接電噴灑游離質譜法鑑別樣品成分的分析方法 中華民國 I 442051 2014.06-2031.05
發明專利 薄層層析電噴灑游離裝置及質譜系統 中華民國 I 452601

2014.09-2032.06

發明專利 旋轉式電噴灑游離裝置、質譜儀,以及質譜分析方法 (RESI) 中華人民共和國 ZL 2010 1 0218273.5

2015.03-2035.03

發明專利 多游離源的質譜游離裝置及質譜分析系統 中華民國 Ι 488216

2015.06-2033.04

發明專利 具有多重固相微萃取探針的熱脫附游離裝置、質譜系統,及質譜分析方法 中華民國 Ι 488215 2015.06-2033.11
發明專利 活體外檢測膀胱癌之方法及系統 中華民國 Ι 487911 2015.06-2030.12
發明專利 熱脫附游離裝置、質譜系統、及質譜分析方法 中華人民共和國 1728739 2015.07-2035.07
發明專利 大氣壓力游離裝置及熱重分析質譜系統 中華民國 I 512782 2015.12-2034.09
發明專利 多游離源的質譜游離裝置及質譜分析系統 中國大陸 2160098 2016.08-2036.08
發明專利 多游離源的質譜游離裝置及質譜分析系統 美國 US9607818B2 2017.03-2034.04
發明專利 以燃燒反應作為游離源的層析質譜裝置 中華民國 I 603372 2017.10-2036.04
發明專利 以燃燒反應生成物游離樣品並藉以近生成物游離樣品並藉以進行質譜分析的方法及裝置 中華民國 I576892 2017.04-2035.01
發明專利 使用多游離源作為連接介面及游離技術的層析質譜裝置 中華民國 I 625524 2018.06-2036.04
發明專利 旋轉式大氣壓力化學游離裝置 歐盟 2367197 2018.08-2031.03
發明專利 即時監測固相胜肽合成反應之質譜系統 歐盟 2633312 2018.11-2031.10

技術移轉

技術名稱 被授權單位  
新式大氣壓力採樣及游離質譜技術 (I)

致寬科技股份有限公司

 

油墨質譜分析方法之開發研究 博士科儀股份有限公司

 

現場化學檢測系統開發 博士科儀股份有限公司

 

熱脫附電噴灑質譜儀 台明科技公司  
多游離源的質譜游離裝置及質譜分析系統 博士科儀股份有限公司  
現場食安檢測儀及其應用 睿健生技股份有限公司及其子公司睿軒檢驗科技股份有限公司  
Electrospray-assisted Laser Desorption Ionization and Ambient Liquid Mass Spectrometry Sources
島津科學儀器股份有限公司