學歷
- 國立中興大學化學學士
(1977-1981)
- 美國蒙大拿州州立大學化學碩士
(有機地球化學) (1985-1988)
- 美國蒙大拿州州立大學化學博士 (分析化學)
(1988-1991)
學術經歷與服務
- 美國賓州州立大學材料科學系博士後研究
(1991-1991)
- 美國蒙大拿州州立大學化學及生化學系訪問副教授
(1995-1996)
- 美國加州大學洛杉磯分校化學及生化學系訪問教授
(2004-2005)
-
國立中山大學化學系副教授
(1991-1998)
- 國立中山大學化學系教授
(1998-迄今)
- 國科會自然處審議委員
(2000-2002)
- 國科會自然處諮議委員
(2003-2005)
- 國科會自然處諮議委員
(2010-2012)
- 台灣質譜學會理事長
(2009-2012)
- 行政院公共工程委員
(2009-迄今)
- 世界質譜基金會執行理事兼亞太區代表
(2009-2016)
- 世界質譜基金會
Thomson Medal Award
Committee 主席
(2011-2012)
- 世界質譜基金會
Curt Brunnée Award
Committee主席
(2013-2014)
- 世界質譜基金會執行理事兼執行祕書
(2014-2015)
- 國立中山大學理學院副院長
(2009-2011)
- 國立中山大學跨領域科學研究中心主任
(2009-2011)
- 國立中山大學科技醫學研究中心主任
(2011-2012)
- 國立中山大學毒藥物暨生醫快篩科技研究中心主任 (2018-迄今)
- Anal. Methods (RSC),副主編
(2009-2012)
- Anal. Methods (RSC),編輯委員
(2013-迄今)
- Mass Spectrom. Let.
(KSMS),編輯委員
(2011-2013)
- Int.
J. Mass spectrom. (Elsevier),諮詢委員會成員
(2012-迄今)
- Current Chromatogr. (Bentham
Science),編輯委員
(2012-迄今)
- Mass Spectrom. (Wiley),編輯委員
(2012-迄今)
- Technology (World Scientific
Publishing Company),編輯委員
(2013-迄今)
- Clin. Mass Spectrom.
(Elsevier),諮詢委員會成員 (2016-迄今)
- J. Am. Soc. Mass Spectrom.
(Springer),編輯委員
(2017-迄今)
- Clin. Chim. Acta.
(Elsevier),專刊編輯委員
(2012-2013)
- Mass Spectrom.
(Wiley),專刊編輯委員
(2013-2014 & 2016-2017)
- J. Food Drug Anal.
(Elsevier),專刊編輯委員
(2018-2019)
- Rapid Commun. Mass Spectrom.
(Wiley),專刊編輯委員
(2019-2020)
獎勵與榮譽
- 最佳碩士論文獎,美國蒙大拿州州立大學 (1988)
- 會議壁報論文首獎,世界人類蛋白質體會議 (HUPO)
(2006)
- 傑出研究獎,行政院國家科學委員會 (2009)
- 英國皇家化學會會士 (FRSC),英國化學會
(2010-迄今)
- 國家創作發明獎,行政院經濟部 (2010)
- 化學技術獎章,中國化學年會 (2011)
- 有庠科技發明獎,遠東財團法人徐有庠先生紀念基金會
(2012)
- 優秀質譜研究學者獎,台灣質譜學會 (TSMS)
(2013)
- 傑出論文審查人獎,美國質譜學會 (ASMS)
(2013)
- 儀器精品獎,第八屆高雄國際儀器展 (2015)
- 傑出技術移轉貢獻獎,行政院科技部 (2016)
- 未來科技突破獎,行政院科技部 (2017)
- 化學最佳論文獎,中國化學會 (2018)
- 台灣質譜學會獎章,台灣質譜學會
(TSMS)
(2019)
- 未來科技突破獎,行政院科技部
(2019)
- 傑出研究獎,行政院科技部 (2020)
- 理學院傑出教學獎,國立中山大學 (2001)
- 研究傑出獎,國立中山大學 (2002)
- 研究傑出獎,國立中山大學 (2006)
- 中山發明獎,國立中山大學 (2007)
- 研究傑出獎,國立中山大學 (2009)
- 產學傑出獎,國立中山大學 (2011)
- 特聘教授 (產學類),國立中山大學 (2011)
- 特聘教授 (學術研究類),國立中山大學
(2011-2014)
- 特聘教授 (產學類),國立中山大學 (2014)
- 特聘教授 (學術研究類),國立中山大學 (2014-2017)
- 產學傑出獎,國立中山大學 (2017)
- 特聘教授 (產學類),國立中山大學 (2017)
- 產學研究績優教師獎,國立中山大學 (2018)
- 學術研究績優教師獎,國立中山大學 (2018)
- 產學研究績優教師獎,國立中山大學 (2019)
- 西灣講座,國立中山大學
(2020)
主辦國內/外會議
- 2013.11.01~11.02
質譜分析技術在臨床醫學檢驗研討會 (2013 Mass
Spectrometry for Clinical
Diagnosis)
- 2014.11.07~11.08
質譜分析技術在臨床醫學檢驗研討會 (2014 Mass
Spectrometry for Clinical
Diagnosis)
- 2015.11.05~11.07
質譜分析技術在臨床醫學檢驗研討會 (2015 Mass
Spectrometry for Clinical
Diagnosis)
- 2016.06.27~06.29
第十三屆台灣質譜學會年會暨學術研討會 (The 13th
Taiwan Society for Mass
Spectrometry Annual Conference)
- 2017.03.27
第一屆快篩技術暨應用研討會
(The 1st Conference on Rapid
Screening Technologies and
Applications)
- 2017.06.12~06.13
質譜在食品安全分析之應用研討會
- 2017.10.12~10.14
質譜分析技術在臨床醫學檢驗研討會 (2017 Mass
Spectrometry for Clinical
Diagnosis)
- 2018.10.25~10.27
質譜分析技術在臨床醫學檢驗研討會
(2018 Mass
Spectrometry for Clinical
Diagnosis)
-
2019.10.17~10.18
食品安全教育/食品安全暨藥物分析之質譜新技術與策略發展研討會
(2019 Symposium for Food Safety
Education/Symposium for New Mass
Spectrometric Technologies and
Strategies for Food Safety and
Drug Analysis)
國際會議邀請演講
2020
-
Invited Speaker, 8th
International Singapore
Lipid Symposium Online, Mar.
2020, Signgapore
- Chair and Invited
Speaker, 2020
Asia-Oceania Mass Spectrometry Conference,
Jan.
2020, Macau, China
2019
- Plenary Speaker, 2019
Mass Spectrometry for Clinical Diagnosis, Sep. 2019,
Ningbo, China
- Invited Speaker, 2019
The Japan Society of Analytical
Chemistry, Sep. 2019, Chiba Ken, Japan
2018
- Plenary Speaker, 2018 Asia HPTLC, Nov. 2018, Bangkok, Thailand
- Invited Speaker, 2018 China Mass
Spectrometry Conference, Nov. 2018,
Guangzhou, China
- Keynote Speaker, National Conference on
Organic Mass Spectrometry, Sep. 2018,
Kaifeng, China
- Session Chair, The 22nd International
Mass Spectrometry Conference, Aug. 2018,
Florence, Italy
- Plenary Speaker, The
5th Ambient Ionization Mass Spectrometry,
Apr. 2018, Xian, China
- Invited Speaker, 14th Asian Conference
on Analytical Sciences, Apr. 2018, Jakarta,
Indonesia
- Invited Speaker, 2018 Mass Spectrometry:
Application for Clinical Laboratory, Jan.
2018, Palm Spring, USA
2017
- Keynote Speaker, 2017 AOMSC, Dec. 2017,
Singapore
- Invited Speaker, 3rd National symposium
on Mass Spectrometry, Dec. 2017, Xia-Men,
China
- Invited Speaker, HPLC 2017 Jeju, Nov.
2017, Jeju Island, Korea
- Invited Speaker, HPTLC 2017, Jul. 2017,
Berlin, Germany
- Invited Speaker, PITTCON 2017, Mar.
2017, Chicago, USA
- Invited Speaker, 2017 Mass Spectrometry:
Application for Clinical Laboratory, Jan.
2017, Palm Spring, USA
2016
- Invited Speaker, International
Conference Global Food Safety &
Antimicrobial Resistance, Nov. 2016,
Shenzhen, China
- Invited Speaker, 2nd International
Conference on Innovations in Mass
Spectrometry, Nov. 2016, Moscow, Russia
- Chairman and Plenary Speaker, the
Ambient Ionization Mass Spectrometry 2016
Conference, Nov. 2016, Guangzhou, China
- Invited Speaker, Biological Mass
Spectrometry Symposium 2016, Oct. 2016,
Tokyo, Japan
- Keynote Speaker, 2016 Korea Society for
Mass Spectrometry, Aug 2016, Seoul, Korea
- Plenary Speaker, Hong Kong Society for
Mass Spectrometry 2016, Jun. 2016, Hong Kong
- Invited Speaker, World Chinese Mass
Spectrometry Conference 2016, Jun. 2016, San
Antonio, U.S.A
- Invited Speaker, PITTCON 2016, Mar.
2016, Atlanta, U.S.A
- Invited Speaker, Mass Spectrometry:
Applications to the Clinical Lab 2016, Feb.
2016, Palm Spring, U.S.A
2015
- Invited Speaker, 5th BCEIA, Oct. 2015,
Beijing, China
- Invited Speaker, HPLC 2015, Sep. 2015,
Beijing, China
- Invited Speaker, Chinese Chemical
Society Second National Symposium on Mass
Spectrometry, Sep. 2015, Hangzho, China
- Invited Speaker, Mass Spectrometry:
Applications to the Clinical Lab 2015, 28th
March~1st April 2015, San Diego, U.S.A
2014
- Invited Speaker, the 17th International
Conference & Exhibition on Analysis &
Testing of Metallurgical Process &
Materials, Jul. 2014, Beijing, China
- Invited Speaker and Session Chair, the
5th Asia and Oceania Mass Spectrometry
Conference, Jul. 2014, Beijing, China
- Keynote Speaker and Session Chair, 2014
HPTLC symposium, Jul. 2014, Lyon, France
- Invited Speaker, PITTCON-2014, Mar.
2014, Chicago, USA
2013
- Lecturer, 1st International Mass
Spectrometry School, Siena University, Sep.
2013, Siena, Italy
- Invited Speaker, 1st International
Conference on Urine Omics (URINOMICS 2013),
Sep. 2013, Lisbon, Portugal
- Invited Speaker and Session Chair, 12th
Asian Conference on Analytical Sciences
(ASIANALYSIS XII), Aug. 2013, Fukuoka, Japan
- Lecturer, International Congress for
Innovation in Chemistry (PERCH-CIC Congress
VIII), May 2013, Pattaya, Thailand
- Invited Speaker, The International
Symposium on SIMS and Related Techniques
Based on Ion-Solid Interaction 2013,Seikei
University, Apr. 2013, Tokyo, Japan
2012
- Invited Speaker, Department of
Biochemistry and Biophysics, Stockholm
University, Nov. 2012, Sweden
- Session and Workshop Chairs, the 29th
International Mass Spectrometry Conference,
Sep. 2012, Kyoto, Japan
- Invited Speaker, Centre an0d Department
of Applied Biology and Chemical Technology,
The Hong Kong Polytechnic University, Aug.
2012, Hong Kong
- Keynote Speaker and Session Chair,
Desorption 2012, Jun. 2012, Castle of
Rauischholzhausen, Marburg, Germany
- Tutorial Lecturer and Invited Speaker,
the 60th American Society for Mass
Spectrometry Conference on Mass Spectrometry
and Allied Topics, May 2012, Vancouver,
Canada
- Invited Speaker, the 1st Analytix-2012,
Mar. 2012, Beijing, China
- Invited Speaker, PITTCON-2012, Mar.
2012, Orlando, USA
2011
- Plenary Speaker, the 12th International
Conference of Ethnopharmacology, Feb. 2012,
Kolkata, India
- Invited Speaker, Molecular Imaging
Workshop, Dec. 2011, Moscow, Russia
- Invited Speaker, the 14th Annual
Conference of Indian Society for Mass
Spectrometry (ISMAS), Nov. 2011, Munnar,
India
- Invited Speaker, International
Conference on Chemistry of Phytopotentials:
Health, Energy and Environmental
Perspectives (CPHEE 2011), Nov. 2011, Agra,
India
- Invited Speaker, the 37th International
Conference of High Performance Liquid
Chromatography, Oct. 2011, Dalian, China
- Invited Speaker, the 14th Beijing
Conference and Exhibition on Instrumental
Analysis (BCEIA), Oct. 2011, Beijing, China
- Session and Workshop Chairs, the 2nd
Asia and Oceania Mass Spectrometry
Conference, Aug. 2011, Busan, Korea
- Invited Speaker, Laboratory of Organic
Chemistry, Eldgenossische Technische
Hochschule (ETH), Jun. 2011, Zurich,
Switzerland
- Session Chair, the 4th International
High Performance Thin Layer Chromatography
Symposium, Jun. 2011, Basel, Switzerland
- Invited Speaker, the 18th Secondary Ion
Mass Spectrometry (SIMS) Symposium, May
2011, Baltimore, USA
2010
- Session Chair and Invited Speaker, the
26th Asilomar Conference, American Society
for Mass Spectrometry, Oct. 2010, Asilomar,
CA, USA
- Keynote Speaker, 2012 Taiwan-China
Bilateral Analytical Chemistry Symposium,
Sep. 2010, Dalian, China
- Plenary Speaker, the 1st AOMSC, Jun.
2010, Tsukuba, Japan
- Invited Speaker, Institute of Chemistry,
Beijing Univ., Jul. 2010, Beijing, China
- Keynote Speaker, the 3rd World Chinese
Mass Spectrometry Conference, Jul. 2010,
Chan-Chun, China
研究興趣
儀器開發
(Instrumentation):
低溫大氣壓游離法
(Low Temperature
Ionization)
|
|
直接電噴灑游離探針 (Direct Electrospray
Probe, DEP) |
|
此技術是將具熱不穩定性及高揮發性之化合物溶於低溫的有機溶劑來抑制其化學活性,並通過低溫管路導入到 ESI 游離源或是二次離子源中進行游離。相較於傳統分析法,此技術可得更佳的不穩定分析物離子訊號。
J.
Mass Spectrom.
(1996, 31, 464-471)
Anal. Chem.
(1996, 68, 1062-1066)
|
|
|
此技術是施加高電壓於一上面置有一微滴之樣品溶液之金屬絲上,金屬絲表面可以是經修飾(如奈米氧化鎢管) 或不經修飾 (如銅絲)。樣品液滴在受到高電場的影響下,會移向質量分析器並在金屬絲尖端產生微電噴灑現象。此技術因組裝簡單,使用材料便宜,樣品更換容易,因此未來可應用於大量樣品數目的分析。
Anal. Chem.(1999, 71,
4413-4417)
Rapid Commun. Mass Spectrom.
(2003, 17, 1709-1713)
Anal. Chem.
(2005, 77, 8170-8173)
Rapid Commun. Mass Spectrom.
(2017, 31, 1-9) |
瞬間熱裂解 (Pyrolysis, Py)
結合融合微滴電噴灑游離法
(Fused-droplet electrospray
ionization, FD-ESI) |
|
電噴灑輔助雷射脫附游離法
(Electrospray-Assisted Laser
Desorption Ionization,
ELDI) |
|
FD-ESI
是我們實驗室所開發出來的大氣壓力游離技術。我們發現在將 Py
結合 FD-ESI 後,我們可以快速偵測到來自高分子材料的熱裂解極性產物,以此方法,我們可以很容易區分各式不同高分子材料。我們發現儘管蛋白質溶液中含有大量的有機或是無機鹽類,但均可在不經任何樣品前處理步驟下,以FD-ESI/MS 直接得到蛋白質離子的訊號。和傳統的ESI/MS 分析結果比較起來,此技術對鹽類的容忍度是前者的100倍以上。
Anal.
Chem.
(2001, 73, 4983-4988)
J. Mass Spectrom.
(2005, 40, 413-415)
Anal. Chem. (2005, 77, 8170-8173)
J. Proteome Res. (2005, 4, 606-612) |
|
|
原理是先以雷射將分析物分子脫附後,再將這些氣相分子導入一以溶劑進行電噴灑區域內,使分析物分子和電噴灑區內之帶電荷溶劑物種反應而產生游離。
Rapid Commun. Mass
Spectrom.
(2005, 19, 3701-3704)
Anal. Chem.
(2008, 80, 6995-7003)
J.
Proteome Res.
(2006, 5, 1108-1116)
Anal. Chem.
(2008, 80, 4845-4852)
Anal. Chim. Acta
(2019, 1066,
69-78)
J. Food Drug Anal.
(2019, 27, 415-427)
|
薄層層析法
(Thin Layer Chromatography, TLC) 結合電噴灑輔助雷射脫附游離法
(Electrospray-Assisted Laser
Desorption Ionization,
ELDI) |
|
雷射誘導聲波脫附 (Laser-Induced Acoustic
Desorption, LIAD) 結合融合微滴電噴灑游離法
(Fused-Droplet Electrospray
Ionization, FD-ESI) |
|
薄層層析法 (Thin Layer Chromatography, TLC)
具有操作簡單、成本低廉、樣品需求量少的特性,且已被廣泛應用在小分子的分析上,與電噴灑的結合可提供直接且快速地沖提物檢測。而電噴灑輔助雷射脫附游離法
(Electrospray
Laser Desorption Ionization,
ELDI)
可在一大氣壓力下,不用複雜的樣品前處理,就直接對樣品進行快速的分析。本研究是利用市售樂高模組具可輕易組裝的優點,並與輸送帶結合,發展成一套高通量且可快速篩選各式成分的TLC-ELDI/MS偵測系統,可針對大量樣品包含藥物、化妝品等進行高效率的分析。
Anal.
Chem.
(2003, 75, 2493-2498)
Anal.
Chem. (2012, 84, 5864-5868) |
|
|
我們將蛋白質溶液塗敷於一鋁金屬薄片上,鋁膜另一面再以雷射照射,以產生震波造成蛋白質分子的脫附,這些被脫附之蛋白質分子在移動到一電噴灑區後,會進一步被電噴灑所游離。我們發現不論樣品是以固體或是液體,均可以此法成功被游離。
Anal.
Chem.
(2009, 81, 868-874)
Anal. Chem.
(2009, 81, 9274-9281)
|
熱脫附電噴灑游離質譜法 (Thermal
Desorption
Electrospray
Ionization,
TD-ESI) |
|
雙電噴灑及大氣壓化學游離法
(Dual Electrospray
and Atmospheric Pressure Chemical
Ionization) |
|
熱脫附電噴灑游離源技術包含了熱脫附裝置和電噴灑離子源,利用一金屬取樣探針沾取或刮取樣品,可於開放空間下分析固體、液體、凝膠及膏狀等型態的樣品,經由加熱區以高溫加熱脫附樣品,並以預先加熱的氮氣作為載流氣體,將分析物攜帶至電噴灑噴霧中產生游離反應,全程只需約
30
秒便可知道結果。具有方便、簡單、快速、即時且感度高等優點。此分析技術提供不同領域的分析者檢測多種不同基質的樣品或於其表面做取樣分析;如尿液中安非他命系列的檢測。
Anal. Chem.
(2013, 85,
8956-8963) |
|
|
開發了結合電噴灑游離(ESI)和大氣壓化學游離(APCI)的雙游離源,同時電離極性和非極性化合物。將高直流電壓施加到在熔融石英毛細管中流動的甲醇溶液中,在以毛細管尖端處產生ESI電噴灑。將高交流電壓施加到連接到玻璃管的環形電極,在玻璃管與不銹鋼管柱間流動的氮氣產生電漿。由於ESI和APCI所需的高電壓是獨立應用和控制的,因此雙離子源可以在僅ESI,僅APCI或ESI
+
APCI模式下運行。通過用連續波長激光或脈衝激光束照射樣品表面將分析物引入ESI和APCI流中。通過將雙ESI+APCI向樣品表面以進行脫附和游離,也可以產生分析物離子。雙離子源中涉及的游離機制包括潘寧電離,離子分子反應和熔滴電噴霧電離。
Anal. Chem. (2015, 87, 1743-1748)
|
火焰誘導大氣壓化學游離法
(Flame-Induced
Atmospheric
Pressure
Chemical
Ionization,
FAPCI) |
|
多通道電噴灑游離法
(Multiple Channel Electrospray
Ionization) |
|
火焰誘導的大氣壓化學游離(FAPCI)是一種溶劑和高電壓組合的
APCI
技術。它利用火焰產生帶電物質,與分析物反應進行游離,並從有機化合物中生成完整的分子離子,與較少的碎片離子。在這項研究中,脫附
FAPCI/MS
被開發用於快速偵測液態,糊狀物和固態的熱穩定有機化合物。通過加熱的噴灑器將液體樣品引入離子源,並且在加熱的噴霧器中形成的分析物與游離源中的帶電物質反應。對於糊狀物和固體樣品分析,會將樣品放置於游離源的火焰附近以進行熱脫附和游離。
J. Am. Soc. Mass Spectrom.
(2017, 28, 1473-1481)
Mass. Spectrom. (2017, 6,
S0065)
Anal. Chem. (2016, 88,
5159-5165)
Rapid Commun. Mass Spectrom.
(2016, 30, 890-896)
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該工作利用連接到多通道電噴灑質譜的流動熱解器檢測高反應性熱解產物環戊二烯基烯酮,環己二烯基烯烴亞胺和乙酰基乙烯酮的質子化分子離子。在流動熱解器中產生的乙烯酮通過氮氣流直接導入七通道電噴霧電離源的中心通道。
同時,通過外部六個通道電噴射含有0.1%三氟乙酸的甲醇溶液。質子化的甲醇離子和由外部六個電噴霧通道產生的帶電液滴促進中性烯酮通過離子-分子反應或吸收隨後質子化而游離。
J. Mass.
Spectrom.
(1997, 32, 247-250)
Anal. Chem.
(1998, 70, 2757-2761)
Anal. Chem.
(2000, 72, 1175-1178) |
旋轉式電噴灑游離法
(Rotating
Electrospray Ionization, RESI) |
|
鋸齒狀薄層層析法結合電噴灑游離質譜法
(Sawtooth
TLC-ESI/MS) |
|
旋轉電噴灑游離質譜法 (Rotating Electrospray Ionization, RESI) 可用來有效提升離子-分子反應
(Ion-Molecule Reaction, IMR)
的效率。其是藉由機動式馬達將不斷噴出帶電液滴的電噴灑游離源進行高速旋轉,並形成一均勻分布的電噴灑噴霧區域,而此現象特別有益於二段式大氣壓力游離質譜法如
FD-ESI, ELDI
及 LIAD-ESI
等技術。除此之外,此技術更可運用在多管式電噴灑游離源上,藉以產生一均勻分布的多管式電噴灑噴霧區域,以克服靜止式因電場干擾所造成的電噴灑不穩定及電噴灑噴霧不均勻等問題。
US8,258,469B2
(美國專利證號)
5193329 (日本專利證號)
1614741 (中國專利證號)
2367197 (歐盟專利證號)
|
|
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開發了一種簡單、便宜的薄層層析 (TLC) 與電噴灑游離質譜
(ESI/MS) 連接設計,用於掃描和鑑定經TLC分離的化合物。將TLC片修剪成兩個鋸齒狀的TLC試片,再以鋸齒狀三角形尖端面對質譜入口,於尖端加一滴甲醇和高電壓以誘導電噴灑的形成,進行試片上分析物的分析。該系統可連續進行高通量分析,在分析第一個尖端中的分析物後,可藉由自動載台移動TLC試片,進行下一個尖端分析物的分析。
Anal. Chim.
Acta (2018,
434, 264-271)
Anal. Chim. Acta (2019, 1049, 1-9)
|
生物醫學
(Biomedicine):
開發以基質輔助雷射脫附飛行時間式游離質譜法
(Matrix-assisted Laser Desorption
Ionization Time-of-Flight Mass
Spectrometry, MALDI-TOF MS)
作為臨床醫學檢驗上診斷特定疾病之生物指標 (蛋白質/胜肽、脂質、代謝物) 的分析技術,並以MALDI-TOF
MS進行分子影像 (Molecular Image),研究各式生物指標在正常及疾病組織中之原位分佈。
|
乳癌指標 (Breast
Cancer)
乳癌是因為乳房乳腺管細胞或是乳小葉細胞產生不正常分裂、繁殖而形成之惡性腫瘤。乳癌除了局部侵犯乳房組織外,更可能轉移到遠處組織,如:肺、骨骼、肝臟、腦等器官,而危害生命安全。 |
|
糞便潛血指標
(Fecal Occult Blood, FOB)
大腸癌的腫瘤或息肉會受到糞便形成過程中的摩擦而流血,血液因而混合在糞便內排出,使得糞便表面含有肉眼看不出來的血液,透過糞便潛血檢查可以早期發現大腸癌或息肉等症狀。 |
|
蛋白尿指標 (Albuminuria)
一般正常尿液因排泄系統構造的關係,尿中蛋白質應小於150毫克
(白蛋白每日排出量應小於30毫克),尿中蛋白質高於標準值時,可能導因於是否有腎臟或心臟方面的疾病。
|
Genomic Medicine, Biomarkers, and
Health Sciences. (2012, 4, 3-6)
Clin. Breast Cancer (2017,
17, 373-381) |
|
Clin. Chim. Acta (2007, 384,
86-92)
Rapid Commun. Mass Spectrom.
(2007, 21, 3311-3316)
J. Dig. Dis. (2013, 14, 474-483)
|
|
Rapid Commun. Mass Spectrom.
(2008, 22, 3754-3760)
Clin. Chim. Acta (2012, 413,
875-882)
Clin. Chim. Acta (2013, 420,
76-81)
Ι
487911
(中華民國專利證號) |
|
缺血性腦中風指標
(Ischemic Stroke)
當腦部血管有局部性的阻塞,使得依靠這條血管供給血液及營養的腦組織受損,造成腦細胞傷害、壞死、局部神經性機能障礙,稱之為缺血性中風,相較於出血性中風,缺血性中風發生機率較高。
|
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精神疾病指標 (Mental Disorders)
是一種以表現在行為、心理活動上的紊亂為主的精神症狀。其原因可能來自外在因素如家庭、社會環境,或是患者自身的生理遺傳因素、神經生化因素等內在原因相互作用所導致的心理活動、行為、及其神經系統功能紊亂,目前臨床診斷較難有具體的疾病指標判別。
|
|
防禦素發炎指標 (Defensins)
人體免疫系統的細胞含有這些防禦素協助殺死被吞噬的細菌,具有很強的抗細菌、真菌和具外套膜病毒作用,其存於嗜中性粒細胞和幾乎所有的上皮細胞。
|
J. Neurol.
(2009, 256, 625-631) |
|
Rapid Commun.
Mass Spectrom. (2009, 23, 589-598)
Clin. Chim. Acta
(2013, 424, 175-181)
Rapid Commun. Mass
Spectrom. (2016, 30,
2645-2649)
Clin Chim Acta
(2017, 473, 75-81)
|
|
Rapid
Commun. Mass
Spectrom. (2009, 23,
3220-3226)
Anal.
Methods (2010, 2, 1934-1940)
Clin. Chim. Acta
(2018, 479, 212-218) |
|
胃幽門桿菌毒性鑑定
(Bacteria Identification)
此菌的螺旋結構及菌上頭的鞭毛,使它能夠鑽入胃黏液而達到胃黏膜上,在黏液與黏膜之間酸度是中性的,而且此細菌本身也可以分泌尿素脢來保護自己,在胃裡面會破壞胃黏膜造成胃發炎,從急性的胃炎到慢性的胃炎它都可以一肩挑,擔任相當吃重的角色,目前已確定胃炎的重要罪魁禍首之一就是它。 |
|
糖化血色素指標 (HbA1c)
糖化血色素是最容易反映血糖濃度的指標之一,血液中的葡萄糖濃度愈高,累積的HbA1c濃度與比率也愈高,且因為紅血球的平均壽命約為3個月,所以測定血液HbA1c的百分比,就可以估計過去120天期間內的平均血糖濃度,目前是糖尿病患者控制血糖的最佳指標。一般來說,HbA1c的正常檢測參考值約為4~6%,國際間採用之糖尿病治療準則是將HbA1c控制在7%以下。 |
|
影像質譜法
(Mass Spectrometry
Imaging , MSI)
此分析方法可利用質譜訊號來分析物質組成,更可了解特定物質在空間中的分布情形。此分析方法之優勢即是不需要使用任何標記或抗體標誌物,即可直接在組織樣品上進行分子影像觀察與研究,並了解其分子空間分布資訊,且能找出與腫瘤發生相關之生化分子。 |
Clin. Chim. Acta (2013, 424,
123-130)
US7,750,291B2 (美國專利證號)
I 340244 (中華民國專利證號)
|
|
Anal.
Chem. (2008, 80, 4845-4852) |
|
Anal Methods
(2010, 2, 1974-1981)
Genomic Medicine, Biomarkers, and
Health Sciences (2012, 4, 3-6)
Clin. Breast Cancer (2017,
17, 373-381) |
急診醫療
(Emergency Medicine):
開發熱脫附式電噴灑游離裝置結合各式高靈敏質譜儀,應用在急診真實案例上。取樣來源可自洗胃液、唾液、皮膚、殘於瓶蓋中快速分析急性中毒之毒性成份,幫助醫師做出正確的治療。
Rapid Commun. Mass Spectrom.
(2015, 29, 163-170)
J. of Mass Spectrom.
(2016, 51, 97-104)
Rapid Commun. Mass Spectrom.
(2016, 30, 1295-303)
Mass Spectrom. (2017, 6,
S0056)
Am. J. Emerg. Med. (2018, 36,
530.e1-530.e5)
Clin Chim Acta (2018, 485, 288-297)
J. Food Drug Anal. (2019, 27, 415-427)
Anal. Chim. Acta
(2019, 1066, 69-78)
Rapid Commun. Mass Spectrom.
(2019, 34
Suppl 1, e8562)
食品安全
(Food Safety):
近年來因食品中毒事件時有所聞,但食品中組合成分複雜,檢測不易。本實驗室開發各種大氣質譜法結合各式質譜儀直接且快速分析目標物,像是檢測易混淆之有毒野菜、野菇中的毒素;針對各式蔬果表面的殘餘農藥;周邊物件表面的塑化劑殘留;化妝品中的防腐劑等,不僅能保障食品的食用安全,更能釐清食品食用後造成健康損害之原因來源。
Rapid Commun. Mass Spectrom.
(2015, 29, 163-170)
Rapid Commun. Mass Spectrom.
(2016, 30, 2315-2322)
Anal. Chim. Acta
(2018, 1039, 65-73)
Int. J. Mass Spectrom.
(2018, 434, 264-271)
Anal. Chim. Acta
(2018, 1039, 65-73)
J. Food Drug Anal. (2019, 27, 415-427)
J. Food
Drug Anal.
(2019, 27, 451-459)
Anal. Methods (2019, 11, 6013-6022)
Anal. Chim. Acta
(2020, 1102, 63-71)
Anal. Chim. Acta
(2020,
1105, 128-138)
刑事鑑識 (Forensic
Science):刑事鑑識一直以來都扮演著輔助案件偵查的重要角色,隨著時代的進步,越來越講究科學辦案,但傳統分析往往受限於時間與成本,導致分析的結果
動輒花上數天。本實驗開發各種大氣質譜法結合各式質譜儀,可直接對物品、不明液體、甚至皮膚上直接進行分析,具有快速、即時、高通量等優點,
為阻嚇犯罪的一大利器。
J. Mass Spectrom.
(2013, 48, 1129-1135)
J. Mass Spectrom. (2014, 49, 445-51)
J. Mass Spectrom.
(2016, 51, 20-27)
Taiwan J. Forensic Medicine
(2016, 8, 28-41)
J. Food Drug Anal.
(2019, 27, 451-459)
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Chen, H. C.; Wu, W. J.; Wu, P.
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J., “Thin Layer
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Cheng, S.
C.; Shiea, J., “Ambient
Ionization Mass Spectrometry - A
Tutorial” Anal. Chim. Acta 2011, 702, 1-15.
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- Huang, M. Z.; Yuan, C. H.;
Cheng, S. C.; Cho, Y. T.; Shiea, J.,
“Ambient Ionization Mass
Spectrometry” Annual
Review Analytical Chemistry,
2010, 3,
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- Shiea, J.; Huang, M. Z.; Hsu,
H. J.; Lee, C. Y.; Yuan, C. H.;
Beech, I.; Sunner, J.,
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Shiea, J., Detecting Large Biomolecules from
High-salt
Solutions by Fused-droplet Electrospray
Ionization Mass
Spectrometry. Anal. Chem. 2002, 74(11), 2465-2469.
(Citation: 131)
- Yuan, C. H.; Shiea, J.,
Sequential Electrospray Analysis
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專書/專刊
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Shiea, J.; Shimma S.; Lai, C. C.
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Issue for 2018 Mass Spectrometry for Clinical
Diagnosis. Rapid Commun. Mass Spectrom. 2020,
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J., “In-situ Detection of
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Shiea, J., A Special
Issue for 2017 Mass Spectrometry for Clinical
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Cheng, S.; Shiea, J.,
“Rapid Characterization
of toxic compounds with
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system”. SciTech Reports., 2018.
Liao, P. C; Shiea, J., A Special Issue for The
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Cheng, S.; Su, H.; Lee, R.
H.; Cai, Y. D.; Lee, C. W.; Shiea,
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Huang, M. Z.; Jhang, S. S.; Shiea, J., “Electrospray Laser
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2020
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Chan, H. C.; Chan, H. C.; Liang, H. J.; Lee,
H. C.; Su, H.; Lee, A. S.; Shiea, J.; Tsai,
W. C.; Ou, T. T.; Wu, C. C.; Chu, C. S.;
Dixon, R. A.; Ke, L. Y.; Yen, J. H.; Chen,
C. H., Role of Low-density Lipoprotein in
Early Vascular Aging Associated With
Systemic Lupus Erythematosus. Arthritis
Rheumatol. 2020, doi: 10.1002/art.41213
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Wang, C. H.; Su, H.;
Chou, J. H.;
Lin,
J. Y.; Huang,
M. Z.; Lee, C. W.;
Shiea, J.,
Multiple
Solid Phase Microextraction Combined with Ambient Mass Spectrometry for
Rapid and Sensitive Detection of Trace
Chemical Compounds in Aqueous Solution. Anal. Chim. Acta 2020, 1107, 101-106.
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Su, H.; Huang, Y. J.; Huang, M. Z.; Lee, Y. T.; Chen,
S. C.; Hung, C. H.; Kuo, C. H.; Wu, M. T.; Shiea,
J., Using Ambient Mass Spectrometry to
Explore the Origins of Phthalate
Contamination in a Mass Spectrometry
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2020, 1105, 128-138.
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Cheng, S. C.; Lee, R. H.; Jeng, J. Y.; Lee, C. W.; Shiea, J., Fast screening of trace multiresidue pesticides on fruit and
vegetable surfaces using ambient ionization
tandem mass spectrometry. Anal. Chim. Acta 2020, 1102, 63-71.
2019
- Shiea, J.; Bhat, S. M.;
Su, H.; Ponnusamy, V. K.; Lee, C. W.; Wang,
C. H., Rapid Quantification of Acetaminophen
in Plasma Using Solid Phase Microextraction
Coupled with Thermal Desorption-Electrospray
Ionization/Mass Spectrometry. Rapid Commun. Mass
Spectrom. 2019, doi:
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- Su, H.; Yeh, I. J.; Wu,
Y. H.; J, Z. H.; Shiea, J.; Lee, C. W., Rapid
identification of organophosphorus
pesticides on contaminated skin and
confirmation of adequate decontamination by
ambient mass spectrometry in emergency
settings. Rapid Commun. Mass
Spectrom. 2019,
doi: 10.1002/rcm.8562. (Special issue)
- Su, H.; Lin Y. P.; Yang,
S. C.; Kuo, C. H.; Wu, D. C.; Shiea, J.;
Lee, C. W., Rapid detection of non-volatile
household pesticides in drained gastric
juice by ambient mass spectrometry for
emergency management. Anal. Chim. Acta
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Y. P.; Jiang, Y. M.; Tsai, Y. H.; Chang, F.
R.; Shiea, J.; Lee, C. W., Rapid
Identification of Herbal Toxins Using Electrospray
Laser Desorption Ionization
Mass Spectrometry for Emergency Care. J. Food
Drug Anal. 2019, 27, 415-427. (Cover image of
special issue)
- Cheng, S. C.; Tsai, Y. D.; Lee, C. W.;
Chen, B. H.; Shiea, J., Direct and Rapid
Characterization of Illicit Drugs in
Adulterated Samples Using Thermal Desorption
Electrospray Ionization Mass Spectrometry.
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(Special
issue)
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Lee, C. W.; Shiea, J., Simple
Interface for Scanning Chemical Compounds on
Developed Thin Layer Chromatography Plates
Using Electrospray Ionization Mass
Spectrometry. Anal. Chim. Acta 2019, 1049,
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Sellappan, S.; Shiea, J.; Ponnusamy, V.
K., Identification and characterization of
unknown degradation impurities in
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formulation using HPLC, ESI-MS and NMR. J. Pharm.
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2018
- Lee, C. W.; Chao, Y. Y.; Shiea, J., Shen
J. H., Lee, H. H.; Chen, B. H., Ambient Mass
Spectrometry for Rapid Diagnosis of
Psychoactive Drugs Overdose in an Unstable
Patient. Am. J. Emerg. Med. 2018, 36(3),
530.e1-530.e5.
- Wu, C. Y.; Lo, L. H.; Su, H.; Shiea, J.,
Detection of α-defensin in Blister Fluids as
Potential Biomarkers for Bullous Pemphigoid
Patients by Matrix-assisted Laser Desorption
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2017
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Comparison of the Performance of
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Wu, J. R.; Wu, M. T., Temporal
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2012
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Y.; Tseng, W. L.; Cheng, C. M.;
Hou, M. F.; Wang, J. M.; Cheng,
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C., The Study of Interferences
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Chang, A. W.; Wu, M. T.,
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2011
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2010
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Huang, B. J.; Wu, C. J.; Chu, P.
Y.; Roffler, S. R.; Cheng, T.
L., Measurement of Poly(ethylene
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2009
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J.; Chang, W. D.; Su, Y. C.;
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2008
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Y. C., Cyclopeptides with
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專利
Category |
TITLE OF THE
INVENTION |
COUNTRY |
PATENT
NUMBER |
PATENT PERIOD |
發明專利 |
ELECTROSPRAY-ASSISTED LASER DESORPTION IONIZATION DEVICE, MASS
SPECTROMETER,
AND METHOD FOR
MASS
SPECTROMETRY
經電噴灑輔助之雷射脫附游離裝置、質譜儀,以及質譜分析方法
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7696475 B2 |
2007.08-2025.08 |
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DETECTION OF NH3
FOR THE PRESENCE
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2008.08-2026.08 |
發明專利 |
MASS SPECTROMETRIC IMAGING METHOD UNDER AMBIENT CONDITIONS USING
ELECTROSPRAY-ASSISTED
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美國 |
US
7,687,772 B2 |
2007.08-2025.08 |
發明專利 |
MASS
SPECTROSCOPIC
REACTION-MONITORING
METHOD |
美國 |
US
7718958 B2 |
2007.08-2025.08 |
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經電噴灑輔助之雷射脫附游離裝置、質譜儀,以及質譜分析方法 |
中華民國 |
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271771 |
2007.01-2026.01 |
發明專利 |
用於偵測一固態樣品中之待測物的質譜分析方法 |
中華民國 |
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334027 |
2010.12-2027.04 |
發明專利 |
用以監控在一液態樣品中之化學反應的質譜方法 |
中華民國 |
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336429 |
2011.01-2027.04 |
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整合式微電灑晶片及其製造方法 |
中華民國 |
275791 |
2005.03-2025.03 |
發明專利 |
RF-DART MASS
SPECTROMETRIC
METHOD AND MASS
SPECTROMETER FOR
ANALYZINGAVAPORIZED
SAMPLE |
美國 |
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2007.08-2027.02 |
發明專利 |
Mass Spectrometric Method and Mass Spectrometer for Analyzing a Vaporized
Sample
用以分析一流液體中所含之氣態分析物的質譜儀,及用以檢驗氨氣的質譜
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美國 |
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2011.04-2027.08
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發明專利 |
用以分析一流體中所含之氣態分析物的質譜儀,及用以檢驗氨氣的質譜分析方法 |
中華民國 |
I
340244 |
2011.04-2027.08 |
發明專利 |
旋轉式電噴灑游離裝置、質譜儀,以及質譜分析方法 |
美國 |
US
8,258,469 B2 |
2012.09-2031.03 |
發明專利 |
旋轉式電噴灑游離裝置、質譜儀,以及質譜分析方法 |
日本 |
5193329 |
2011.03-2031.03
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發明專利 |
電噴灑游離裝置、質譜儀,以及質譜分析方法 |
中華民國 |
430322 |
2014.03-2031.03 |
發明專利 |
旋轉式脫附電噴灑游離裝置及質譜系統 |
中華民國 |
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435367 |
2014.04-2030.09 |
發明專利 |
旋轉式大氣壓力化學游離裝置 |
中華民國 |
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435365 |
2014.04-2030.10 |
發明專利 |
經電噴灑輔助之熱脫附游離裝置、質譜儀、以及質譜分析方法 |
中華民國 |
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435366 |
2014.04-2031.05 |
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以直接電噴灑游離質譜法鑑別樣品成分的分析方法 |
中華民國 |
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薄層層析電噴灑游離裝置及質譜系統 |
中華民國 |
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452601 |
2014.09-2032.06
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旋轉式電噴灑游離裝置、質譜儀,以及質譜分析方法 (RESI) |
中華人民共和國 |
ZL
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發明專利 |
多游離源的質譜游離裝置及質譜分析系統 |
中華民國 |
Ι
488216 |
2015.06-2033.04
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發明專利 |
具有多重固相微萃取探針的熱脫附游離裝置、質譜系統,及質譜分析方法 |
中華民國 |
Ι
488215 |
2015.06-2033.11 |
發明專利 |
活體外檢測膀胱癌之方法及系統 |
中華民國 |
Ι
487911 |
2015.06-2030.12 |
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熱脫附游離裝置、質譜系統、及質譜分析方法 |
中華人民共和國 |
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大氣壓力游離裝置及熱重分析質譜系統 |
中華民國 |
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512782 |
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多游離源的質譜游離裝置及質譜分析系統 |
美國 |
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旋轉式大氣壓力化學游離裝置 |
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